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Ben Si Said Hassan
Department of Pharmacy, Mouloud Mammeri University, Tizi Ouzou, Algeria
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Research Article
Development, Validation of a Method for the Determination of Oxaluria by UV HPLC after Derivatisation with O-Phenyldiamine
Author(s): Belkaid Nawal* and Ben Si Said Hassan
Introduction: Urinary oxalate is recognized as an important biomarker for the diagnosis and monitoring of hyperoxaluria responsible for oxalocalcic lithiasis. Objective: The main objective of our study, carried out in the Biochemistry and Analytical Chemistry Laboratory of the Pharmacy Department at the Université Mouloud Mammeri Tizi-Ouzou (UMMTO), is to develop and validate a method for the determination of urinary oxalate by reverse-phase HPLC, to be applied routinely in the biochemistry laboratory for the benefit of patients. Method: Determination of urinary oxalate by reverse-phase HPLC (C18) after derivatization with ophenylenediamine. Validation according to SFSTP 2006 protocol V2 followed by stability study and establishment of internal quality controls. Results: The method.. View more»