Biochemistry & Analytical Biochemistry

Abstract

Development, Validation of a Method for the Determination of Oxaluria by UV HPLC after Derivatisation with O-Phenyldiamine

Belkaid Nawal*, Ben Si Said Hassan

Introduction: Urinary oxalate is recognized as an important biomarker for the diagnosis and monitoring of hyperoxaluria responsible for oxalocalcic lithiasis.

Objective: The main objective of our study, carried out in the Biochemistry and Analytical Chemistry Laboratory of the Pharmacy Department at the Université Mouloud Mammeri Tizi-Ouzou (UMMTO), is to develop and validate a method for the determination of urinary oxalate by reverse-phase HPLC, to be applied routinely in the biochemistry laboratory for the benefit of patients.

Method: Determination of urinary oxalate by reverse-phase HPLC (C18) after derivatization with ophenylenediamine. Validation according to SFSTP 2006 protocol V2 followed by stability study and establishment of internal quality controls.

Results: The method was validated with analytical specificity within the quantification limit (12.5; 250) mg/L with coefficients of variation ≤ 6%, relative bias ≤ 7%, regression is linear with a linear coefficient of determination of 99.5%, satisfactory sample stability.

Conclusion: Our method is validated and can be used for routine oxaluria determination.

Published Date: 2025-06-18; Received Date: 2024-08-26