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Single Cell Gene Expression

Gene Expression in individual cells varies from tissue or culture sample according to Cell cycle, genetic, epigenetic and stochastic differences. Single Cell Differentiation is neglected in in the analysis of the functional consequences of genetic variation. The expression of 92 genes affected by signaling in 1,440 single cells from 15 individuals is measured individuals to associate single-nucleotide polymorphisms (SNPs) with gene-expression phenotypes, while accounting for stochastic and cell-cycle differences between cells.Complex tissues may contain very small cell subpopulations with unique gene expression profiles, and averaging gene expression results from many cells can mask subtle, important changes occurring within single cells.Technical advances in single cell isolation, such as laser capture microdissection and fluorescence-based cell sorting, provide the means to capture and recover individual cells, but the detection of minute starting amounts of RNA remains a significant challenge to studying gene expression in these tiny samples, particularly when analysis of multiple targets is required.

Related Journals of Single Cell Gene Expression

Journal of Molecular and Genetic Medicine, Journal of Phylogenetics & Evolutionary Biology, Immunogenetics: Open Access, Advancements in Genetic Engineering, Critical Reviews in Eukaryotic Gene Expression, Gene Expression Patterns, Gene Expression, Genes to Cells, Cell Stem Cell

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