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Dr. Sangita Singh
Dr. Sangita Singh
Dr. Sangita Singh
Research Scientist
DuPont Knowledge Center
India
India
Biography
Dr. Sangita Singh, Research Scientist at DuPont Knowledge Center Hyderabad Area, India. She is Dorctorate from University of Michigan Medical School. More than five years of experience in protein expression as well as purification, enzymatic assays and bioanalytical techniques. She has totla 7 publication invarious journals. Dr. Sangita Singh research interest includes Protein expression in E.Coli, multi-step protein purification, FPLC. Enzymatic assay using radiolabeled substrates. Expert in UV-Visible, HPLC,fluorescence, stopped-flow, EPR and kinetic data fitting using Sigmaplot.,Molecular cloning, protein sequence and structure analysis, Generation of stable cell lines, transfection, cell based assay, metabolic labeling, western blotting, immunoprecipitation, antibody purification, subcellular fractionation
Research Interest
Protein expression in E.Coli, multi-step protein purification, FPLC. Enzymatic assay using radiolabeled substrates. Expert in UV-Visible, HPLC,fluorescence, stopped-flow, EPR and kinetic data fitting using Sigmaplot.,Molecular cloning, protein sequence and structure analysis, Generation of stable cell lines, transfection, cell based assay, metabolic labeling, western blotting, immunoprecipitation, antibody purification, subcellular fractionation