Journal of Clinical & Experimental Pharmacology

Valuation of toxicity assay of crude drug; Clerodendrum infortunatum L

2^nd International Conference and Exhibition on Pharmacology and Ethnopharmacology

May 02-04, 2016 Chicago, USA

Talukdar Muhammad Waliullah and Akter Mst Yeasmin

Shizuoka University, Japan

Posters & Accepted Abstracts: Clin Exp Pharmacol

Abstract:

Objective: To learn a scientific knowledge, this study was examined for evaluating anticancer, anti-microbial and pharmacological activities of natural products and the estimation of cytotoxic potency using root, leaf and stem of ethanol and ethyl acetate extracts of Clerodendrum infortunatum (Verbenaceae) due to randomly use in customary and traditional medicine to cure common ailments such as intestinal disorder, diarrhea, tuberculosis and respiratory problems etc. Methods: The in vitro application was carried out by using disc diffusion, micro broth dilution and serial dilution techniques against clinically important life threatening organisms and the bench top bioassay method using brine shrimp lethality bioassay. Results: All the extracts unveiled significant inhibitory activity over the bacteria and fungus comparable to the standard drug tetracycline and fluconazole. The maximum average diameter zone of inhibition was recorded to bacterial strains against B. megaterium, S. typhi, K. pneumoniae and to fungi against A. niger and C. albicans. The MIC values of ethanol leaf extract were determined 64μg/ml to B. megaterium, S. typhi and K. pneumoniae; 128 μg/ml to S. aureus, S.-β�??haemolyticus and E. coli and interestingly, these extracts were also found to be conspicuously lethal precisely the LC50 value of root of ethanol fraction was 20.845 ppm compare to the standard drug tetracycline was 14.675 ppm to brine shrimp nauplii indicating that the extracts are biologically active. Conclusion: The findings evidently appear promising antibacterial and antifungal along with cytotoxic properties of C. infortunatum against antagonistic pathogens. This study serves as a potential template for the implications of these results for bioactivity and drug discovery.

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