Journal of Clinical & Experimental Pharmacology

Validated HPTLC method for quantification of luteolin and apigenin in Premna mucronata Roxb., Verbenaceae

2^nd International Conference and Exhibition on Pharmacology and Ethnopharmacology

May 02-04, 2016 Chicago, USA

Nayan G Patel

Dharmsinh Desai University, India

Scientific Tracks Abstracts: Clin Exp Pharmacol

Abstract:

A simple, rapid and precise high-performance thin-layer chromatographic method was developed for quantitative estimation of luteolin and apigenin in Premna mucronata Roxb., family Verbenaceae. Separation was performed on silica gel 60 F254 HPTLC plates using Toluene: Ethyl acetate: Formic acid (6:4:0.3) as mobile phase for elution of markers from extract. The determination was carried out in fluorescence mode using densitometric absorbance-reflection mode at 366 nm for both luteolin and apigenin. The methanolic extract of Premna mucronata was found to contain 10.2 mg/g% luteolin and 0.165 mg/g% of apigenin. The method was validated in terms of linearity, LOD and LOQ, accuracy, precision and specificity. The calibration curve was found to be linear between 200-1000 ng/band for luteolin and 50-250 ng/band for apigenin. For luteolin and apigenin, the limit of detection was found to be 508.13 ng/band and 7.97 ng/band while the limit of quantitation was found to be 1539.78 ng/band and 24.155 ng/band, respectively. This developed validated method is capable of quantifying and resolving luteolin and apigenin in its plant raw-material.

Biography :

Nayan G Patel has completed his Postgraduate in Pharmacology from Anand Pharmacy College, Anand, Gujarat and pursuing his PhD in Dharmsinh Desai University. He is serving as an Assistant Professor at Faculty of Pharmacy, Dharmsinh Desai University, Nadiad, Gujarat since last two years and six months. The area of his interest is silver nanoparticles and its implications in various diseases. He also has a very good hands on pre-clinical animal studies with more than 10 types of research completed by him. He has published 4 papers in reputed journals and two still under communication. He is the recipent for grant of Rs. 50 lakhs from Department of Biotechnology, India this year for his proof of concept on research proposal related to use of silver nanoparticles in burns.

Email: nayan_pharma87@yahoo.in