Oral Health and Dental Management

The effect of mesenchymal stem cells application on bone quality during normal and rapid rate distraction osteogenesis (An experimental study)

4^th Asia Pacific Congress & Expo on Dental and Oral Health

July 27-29, 2015 Brisbane, Australia

Alaa Hanna Al Hammoud

Posters-Accepted Abstracts: Oral Health Dent Manag

Abstract:

Regenerate bone quality associated with Distraction Osteogenesis (DO) is one of the drawbacks associated with rapid distraction rate. Mesenchymal stem cells (MSCs) carries hope in improving bone regenerate quality. The purpose of this study was to investigate the effect of mesenchymal stem cell application in the improvement of bone quality of bone regenerate with rapid and normal rate distraction osteogenesis. This study was conducted on 28 goats equally divided into four groups. Group A: normal rate DO (1 mm/day) aided by MSCs, group B: rapid rate DO (2 mm/day) aided by MSCs, Group C: normal rate DO without MSCs and Group D: rapid rate DO without MSCs. All groups had 30 days of consolidation followed by sacrifice Samples were dissected from the animal cadavers to be assessed histologically by measuring trabecular bone thickness of H&E stained samples and osteiod bone percentage measurement of Masson Trichrome stained samples. Histomorphometric evaluation showed increase trabecular bone thickness in group A and group B with high statistical significance compared to group C and Group D. The osteoid percentage was higher in the mesenchymal stem cell treated samples with high statistical significance. MSCs application showed promising results in improving regenerate quality with normal and rapid distraction rates. MSCs application associated with rapid rate enhanced regenerate quality over the rapid rate alone. Higher percentage of osteiod has indicates rapid healing of mesenchymal stem cells treated groups. MSCs improved bone quality of regenerate and compensated the bad effect of rapid distraction.