Journal of Food Processing & Technology

The decoration of selenium nanoparticle with Dictyophora Indusiata polysaccharide and its anticancer Activity

6^th Global Summit and Expo on Food & Beverages

August 03-05, 2015 Orlando-FL, USA

Jiaoyan Ren 1, 2, Wenzhen Liao1, Zehua Lin1 and Joe M Regenstein2

Posters-Accepted Abstracts: J Food Process Technol

Abstract:

Selenium nanoparticles (SeNPs) have been proved to have biological activities along with low toxicity, but are quite unstable and poorly taken up by cells. In this paper, a new polysaccharide (DP1), purified from Dictyophora indusiata, was used to functionalize SeNPs to form high stable nanocomposites DP1-SeNPs with an average diameter of 89.14 nm. DP1-SeNPs showed notable antiproliferative effect on human cancer cell lines (HepG2, MCF-7, SGC-7901, A549, Hela and PC3). The antiproliferative effect of DP1-SeNPs was associated with nuclear condensation, DNA cleavage, S phase cell arrest. With the treatment of DP1-SeNPs, caspase-3, 8, 9 were triggered in a dose-dependent manner in HepG2 cells. Also, the expression level of FADD was increased dramatically, implying that DP1-SeNPs induced apoptotic pathways were mainly through the activation of FADD and caspase-3, 8, 9. Furthermore, ROS over generation and mitochondrial dysfunction were also involved in DP1-SeNPs-induced apoptosis. Therefore, the underlying antiproliferative activity mechanism of DP1-SeNPs was both via death receptor-mediated and mitochondria-mediated apoptotic pathways. This work could eventually lead to development of anti-cancer nutraceutical supplements using natural materials of food origin.