Clinical Microbiology: Open Access

The antibacterial effect of ethanolic extraction of Pimpinella anisum L. in inhibition of Mycobacterium bovis compared with ethambutol

7^th Euro Global Summit on Clinical Microbiology and Mycotoxins

February 27-28, 2017 Amsterdam, Netherlands

Azimi Leila

Mofid Children Hospital-Shahid Beheshti University of Medical Sciences, Iran

Posters & Accepted Abstracts: Clin Microbiol

Abstract:

Introduction: Botanical medicine is available and has a little side effects and toxic effects compared with chemical drugs. In this research, we consider effects of Pimpinella anisum L. with ethambutol in growth of Mycobacterium bovis. Methods & Materials: Ethanolic extract was prepared by percolation method. 0.5 Mc Farland of Mycobacterium bovis from Pasteur institute BCG vaccine was cultured on 24 wells plates contain middle brook 7H10 in different dilution. All plates were incubated at 37°C and examined after 1 week. Results: Most effective concentration of extraction of Pimpinella anisum L. was 400 mg/m that had inhibition growth effect as like as MIC of ethambutol. We didnâ??t have any inhibition zone with disks containing ethanolic extraction by disk diffusion method because extraction couldnâ??t diffuse across the disk. Conclusion: As extraction of Pimpinella anisum L. with 400mg/ml concentration caused to inhibit the growth of Mycobacterium bovis, we suggest to isolate and consider antibacterial effect of matter in whole extraction by HPLC in the further studies.

Biography :

Email: leilaazimi1982@gmail.com