Clinical Microbiology: Open Access

Synthesis of isomalto-oligosaccharides by cell bound transglucosidase of debaryomyces hansenii

Joint Event on 25^th Asia Pacific Biotechnology Congress & 3^rd International Conference on Medical and Clinical Microbiology

May 01-02, 2019 Kyoto, Japan

P Rameshthangam and R Saravanan

Alagappa University, India

Scientific Tracks Abstracts: Clin Microbiol

Abstract:

Transglucosidase is responsible for the production of Isomalto-Oligosaccharides (IMO), which is an important functional food ingredient to promote the growth of beneficial intestinal bacteria. It catalyzes both hydrolytic and transfer reactions (transglucosylation) converting the α-1, 4 linkages to α-1, 6 linkages by bond cleavage and bond formation which leads to formation of higher oligosaccharides. In addition, other health benefits have been reported such as stimulation of intestinal mobility, low glycemic index, low calorie sweetener and mineral absorption. The main objective of the present study is to screen and isolate the transglucosidase producers from soil that has capability to produce isomalto-oligosaccharides. After several screening in maltose enriched media and biochemical characterization of isolates, SCY204 (yeast strain) was isolated and identified as Debaryomyces hansenii among 70 microbial strains which exhibit maximum transglucosidase activity by conversion of maltose to product. A mixture of isomaltose, panose, isomaltotriose and isomaltotetraose were obtained as determined by High Performance Liquid Chromatography (HPLC). Further studies also carried out to optimize the parameters like different media composition, various pH, temperature, metal ions, substrate and biomass concentration to obtain maximum transglucosidase activity through biotransformation. With optimized parameters of temperature (37 °C), substrate (30% w/v) and biomass concentration (8% w/v), more than 98% of maltose utilization with the production of IMO (>60% purity) with >80% yield were achieved through biotransformation.

Biography :

P Rameshthangam has completed his PhD in Biotechnology from University of Madras, India. He is currently working as an Assistant Professor at Alagappa University, India. His research activities is focused on to fabricate the chitin nanoparticles and its composites from natural resources and to explore its enormous biological and environmental applications. His current research work focuses is involved in the synthesis of transglucosidase producing microbes. He has published his research findings in various international SCI journals and also one Indian patent.

E-mail: rameshthangam@alagappauniversity.ac.in