Journal of Clinical & Experimental Pharmacology

Syntheses and characterization of Pseudocerastes persicus snake venom nanoparticles by alginate and alginate chitosan in vitro

Co-organized Event International Conference on Toxicology and Clinical Pharmacology & 2^nd International Conference on Generic Drugs and Biosimilars

December 14-16, 2017 Rome, Italy

Fatemeh Abedini, Hamid Reza Goudarzi, Jamal Rashidiyani, Hossein Hosseinkhani, Ali Nazari Shirvan, Mohammad Ebrahimi, Shahrokh Navidpour and Roozbeh Fallahi

Razi Technology Incubator �?? Razi VSRI, Iran
Razi Vaccine and Serum Research Institute, Iran
Baqiyatallah University of Medical Sciences, Iran
National Taiwan University of Science and Technology, Taiwan

Posters & Accepted Abstracts: Clin Exp Pharmacol

Abstract:

Purpose: There are many species of venomous snakes that belong to the Viperidae family. Pseudocerastes or Persian horned viper is a genus of venomous vipers endemic to the Asia particularly in Iran. Pseudocerastes persicus snakebites are prevalent in semi-deserts area. Since 1960, the production of Snake antivenom greatly diminishes the risk of mortality in Iran. Recently, the idea of using nanoparticles made from natural polymers has provoked great interests among researchers. Among them, chitosan (CS) and alginate (ALG) together or separately are commonly used polymers in the pharmaceutical industry. The 2-DE assay of the Viper venom showed different degrees of complexity in polypeptide composition. The objective of our study was to compare Pseudocerastes persicus venom/Alginate nanoparticles to Alginate Chitosan Pseudocerastes persicus venom nanoparticles in vitro. Methods: Nanoparticles characterizations were done by measuring the particle size, zeta potential, encapsulation efficiency, loading capacities, release profile, and SDS-Page. The zeta potential and particle size of nanoparticles were studied by the zeta potential analyzer and dynamic light scattering (DLS). Encapsulation efficiency, loading capacities and release profile were obtained by using Bradford assay. Results: In vitro characterization of ALG/ snake venom and ALG/CS/ snake venom nanoparticles demonstrated that ALG/ CS/ snake venom nanoparticles showed suitable size (147 nm), PDI (PDI <0.4), zeta potential (+31.2 mV), loading capacity (100%) and best release profile in compared to ALG/ snake venom nanoparticles. ALG/snake venom nanoparticles showed very small size and negative zeta potential that was not suitable for using as antivenom. Our observations showed that about 100% of the venom loaded in ALG/CS nanoparticles were released within 96 hours of incubation, whereas ALG/ snake venom nanoparticles were released within 72 hours. Conclusion: It is suggested that ALG/CS/ snake venom nanoparticles that was synthesized in our study with positive charge, suitable size and best loading capacity may enhance the levels of antibodies in the body of a horse. Further investigation need to carry out in vivo to evaluate the level of antibody by administration of two kinds of nanoparticles.