Clinical Microbiology: Open Access

Studying of antagonistic activity and 2-phenylethanol effects on yeasts Kluyveromyces marxianus

3^rd International Conference on Clinical Microbiology & Microbial Genomics

September 24-26, 2014 Valencia Convention Centre, Spain

Mameeva O G

Accepted Abstracts: Clin Microbial

Abstract:

PEA is higher aromatic alcohol with a rose-like odor and one of the most useful fragrance chemicals in perfume and cosmetic industry. Also PEA has been used for many years as a bactericide in pharmaceutical preparations. Yeasts K. marxianus is receiving increasing interest for development of biotechnological production processes for 2-phenylethanol. In general, cell membrane seems to be an important target site for PEA toxicity. In present study we investigated the antagonistic activity of yeasts K. marxianus UCM Y-301 and UCM Y-305, PEA toxicity on yeast cells and changes in cell fatty acids composition under the different growth conditions. Antagonistic properties of the strains were studied using three kinds of agar media: meat-extract, mono-nutrient medium (0.75 % yeast extract) and multi-nutrient medium (9% sucrose, 1.5% yeast extract, 0.2 g/L L-phenylalanine). The highest antagonistic activity was observed for strain K. marxianus UCM Y-305 growth on meatextract agar and multi-nutrient medium (zones of growth inhibition of the test-bacteria were from 2.5 up to 6.0 mm and from 1.6 mm up to 4.2 mm, respectively). The high concentrations of PEA inhibit yeast growth and their sensitivity towards these alcohols was strain specific. It has been shown that the fatty acid composition of K. marxianus UCM Y-301 and UCM Y-305 directly depends on the different stress conditions such as the concentration of produced PEA, etc. It has been established that the presence of the oleic acid in high concentrations and the degree of unsaturation in the yeast cell membrane increased stress tolerance to PEA.

Biography :

Mameeva O G has completed her PhD in microbiology in 2004 and currently working as a senior researcher in the Department of physiology of industrial microorganisms in the Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine. She has over eleven years of experience in a laboratory setting. She has published more than 17 papers in reputed journals. The main research interest are microbial and biochemical technology.