Study of cytokine profiles in HIV/AIDS patients in India
4th International Conference on Clinical Microbiology and Microbial Genomics
October 05-07, 2015 Philadelphia, USA

Megh S Dhakad, Ravinder Kaur, Ritu Goyal, Preena Bhalla and Richa Dewan

Maulana Azad Medical College & Associated Lok Nayak Hospitals, India
Lady Hardinge Medical College & Associated Hospitals, India

Posters-Accepted Abstracts: Clin Microbiol

Abstract:

Objectives: A switch from a T helper 1 (Th1) to a Th2 cytokine has been proposed as an important factor in progression of HIV infection to AIDS. Hence our objectives were to analyze the levels of Th1 (IL-2, IFN-�³) and Th2 (IL-4, IL-10) cytokines and their correlation with clinical and immunological profiles in symptomatic and asymptomatic HIV/AIDS patients. Methods: We studied four group of patients; symptomatic HIV positive (n=234) (Case group), asymptomatic HIV positive (n=50), symptomatic HIV negative (n=50) and healthy controls (n=50). CD4+T cell count was determined by flow cytometry using Fluorescent Activated Cell Sorter Count system. Quantitative determination of cytokines (Th1 subtype: IL-2, IFN-�³ & Th2 subtype: IL-4, IL-10) was done by ELISA. Results: Patients (08-68 years) had CD4+T cell counts ranged from 16-1033cells/�¼l. The median CD4+T cell count was 204.50cells/ �¼l and the mean was 265.48cells/�¼l. The concentration of IL-2 and IFN-�³ were significantly lower in case group compared to asymptomatic HIV patients (P<0.001) while the IL-4 in symptomatic HIV negative was higher than healthy controls and case group compared to asymptomatic HIV patients. Concentration of IL-10 was also higher in case group compared to asymptomatic HIV patients. A positive correlation was found between IL-4, IFN-�³, IL-2 with CD4+T cells and a negative correlation between IL-10 & CD4+T cells among the Case group. Conclusions: A decline in type-1 cytokines (IFN-�³, IL-2) and rise in type-2 cytokines (IL-4, IL-10) was observed in symptomatic HIV/AIDS patients in comparison to asymptomatic HIV/AIDS patients suggesting a shift from Th1 to Th2 type cytokine response.

Biography :

Email: megh_singh61@yahoo.com