Clinical Microbiology: Open Access

S. aureus modulates host innate immune response by regulating the expression of type 1 IFN

4^th International Conference on Clinical Microbiology and Microbial Genomics

October 05-07, 2015 Philadelphia, USA

Rochika Singh

Indian Institute of Advanced Research, India

Posters-Accepted Abstracts: Clin Microbiol

Abstract:

The complications due to infection bacterial and viral have a major impact on the clinical course of patients with chronic lymphocytic leukemia inspite of rapid development in therapeutic approaches to this disease and supportive care. Various infections are known to be common including during chemotherapy specifically Staphylococcus aureus, Streptococcus pneumonia, Hemophilus influenza, Escherichia coli, Klebsiella pneumonia and Pseudomonas aeruginosa are frequent isolates. The emergence of S. aureus is becoming major concern due to emergence of antibiotic resistant strains. The recent developments from our lab had shown that it is major cause of dysregulation of systematic innate immune response. In the current study we report the intracellular survival and its role in innate immune response.The S. aureus infected HEK 293 T cells gradually induced type 1 IFN after 6hr infection of S. aureus showed decreased almost 90% after 24 hr S. aureus infection. The HEK 293 T cells were infected with S. aureus for 0hr, 4hr, 6hr and 24hr. The expression of type-1(IFNα) induction was checked through RT-PCR. The gradual increase found in IFNα induction. Another study showed that the stressed S. aureus cells at 95°C induce type 1IFN rather than given treatment of 65°C in host cells.The HEK 293 T cells were infected with S. aureus and transfected with MITA, MAVS, NLRX1 and IKKε the molecules involved in induction of type 1 IFN. The western blot analysis showed that IFN was more induced by MAVS in comparison of MITA and NLRX1 at 6 hr infection. Another analysis showed that type 1 IFN less induced in IKKε transfected HEK 293T cells taken MAVS as control. The truncated region of all the molecules sh-MITA, sh-MAVS, sh-NLRX1 and shIKKε associated with type 1 IFN induction via different pathway were checked for INF induction associated with % C.F.U concludes S. aureus infection in host cells. It is observed that sh-MAVS increased the C.F.U. 130% in comparison of sh-MITA and shNLRX1 showed decreased 70% and 40% in C.F.U. count. The count of S. aureus cells found almost same in shIKKε transfected HEK 293 T cells when compared with shNLRX1 taken sh-MAVS as control. The study here strongly suggests that S. aureus can survive intra-cellularly and may be cause of chronic inflammatory conditions. This may have important implication during hematological malignancies. The long term goal will be tackle the host innate immune response to tackle S. aureus infection during these malignancies.

Biography :

Email: rochikaraj@yahoo.com