Clinical Microbiology: Open Access

Role of rv0518 and rv1075c genes of Mycobacterium tuberculosis in cell wall remodeling

Joint Event on 8^th Annual Congress on CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES & 13^th World Congress on VIROLOGY, INFECTIONS AND OUTBREAKS

December 05-06, 2018 | Vancouver, Canada

Jashandeep Kaur and Jagdeep Kaur

Panjab University, India

Scientific Tracks Abstracts: Clin Microbiol

Abstract:

Tuberculosis (TB) is one of the top ten causes of death worldwide and has existed for millennia. TB is caused by Mycobacterium tuberculosis. India alone accounted for one-fourth global TB burden and the reason behind this, is HIV co-infection, the emergence of multi-drug resistant (MDR) and extensively drug-resistant (XDR) strains. In TB database, rv0518 and rv1075c genes have been annotated as hypothetical and need characterization for assigning a role. Rv0518 and Rv1075c have been annotated as GDSL lipase. In this study, we have cloned rv0518 and rv1075c gene in pVV16 shuttle vector, expressed in M. smegmatis mc2155 strain. The expression of rv0518 was significantly up-regulated under nutrient stress and rv1075c gets up-regulated during both acidic and nutritive stress in M. tuberculosis. Their expression also enhanced the total lipid content of the cells. The various components of the mycolic acid layer were found to be enhanced with their expression, resulted in remodeling of the mycobacterial cell wall. In addition, rv0518 and rv1075c expression led to the morphological changes in the colonies and biofilm formation capability of M. smegmatis. The expression of rv0518 and rv1075c provided resistance to various cell wall stresses like SDS, lysozyme and antibiotics to M. smegmatis.

Biography :

Jashandeep Kaur is PhD scholar in Department of Biotechnology, Panjab University, Chandigarh, India. She has been working on deciphering the role of lipolytic enzymes in the life-cycle of Mycobacterium tuberculosis under the supervision of Prof Jagdeep Kaur. Her expertise is in molecular biology, protein biochemistry and animal tissue culture.

E-mail: jashandhanoa89@gmail.com