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Reclassification of Enterobacter sakazakii isolates and characterization of their transmission and virulence related factors
International Conference on Food Microbiology
August 08-10, 2016 Birmingham, UK
Purni Chalukya Kumari Wickramasinghe
Chonbuk National University, South Korea
Posters & Accepted Abstracts: J Food Process Technol
Abstract:
Cronobacter (previously known as Enterobacter sakazakii) is a diverse bacterial genus consisting of seven species: C. sakazakii, C. malonaticus, C. turicensis, C. universalis, C. muytjensii, C. dublinensis and C. condimenti. In this study the taxonomic relationship and pathogenicity of Cronobacter spp. was further investigated in relation to factors that are related to their transmission and virulence. A total of 35 isolates previously identified as E. sakazakii and 7 known Cronobacter reference strains were reclassified on the basis of biochemical tests, 16S rDNA gene sequences and multilocus sequence typing results. After the initial classification of the isolates, further characterization of each isolate was carried out with the use of multiple assays including motility assay, biofilm assay, ELISA assay with OmpA and OmpX antibodies, adhesion assay and invasion assay using 4 to 7 day old human intestinal epithelial Caco-2 cells. Virulence and transmission related characteristics were expressed at different levels among all the isolates studied, however C. sakazakii, C. malonaticus and C. turicensis notably had the highest expression of each of the respective phenotypes. All the virulence and transmission related factors were expressed in a species dependent manner even though a clear relationship between the expressions of these characters could not be established with their ST type allocation. However the existence of novel positive correlationships between each of the phenotypes-biofilm formation, motility, expression of OmpA and OmpX, adhesion and invasion to 4 to 7 day old human Caco-2 cells was also elucidated for the first time in this study.
Biography :
Email: purniwick@yahoo.com