Clinical Microbiology: Open Access

Prevalence and rapid diagnosis of acute bacterial meningitis in childhood in Bangladesh

Joint Event on 8^th Annual Congress on CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES & 13^th World Congress on VIROLOGY, INFECTIONS AND OUTBREAKS

December 05-06, 2018 | Vancouver, Canada

Mohammad Zakerin Abedin

University of Dhaka, Bangladesh

Scientific Tracks Abstracts: Clin Microbiol

Abstract:

An attempt was made to analyze the cerebrospinal fluid (CSF) profile and to isolate and identify aetiological agents from the specimens from children with suspected acute bacterial meningitis. Among total 79 samples, 65(82.3%) were crystal clear, 9(11.4%) were moderately turbid, 2(2.5%) highly turbid and remaining 3(3.8%) were high blood mixed. The total cell (leucocyte) count of the CSF was proportionate to the turbidity. In case of crystal-clear CSF???s, total leucocyte counts were normally ranging from 0 to 700 per mm3 with predominant lymphocytes. Moderately turbid fluid showed 200 to 2,000 cells per mm3 and highly turbid fluid and highly blood mixed showed more than 40,000 cells per mm3. In the later cases, differential counts demonstrated polymorphonuclear predominance. In 65 cases whose CSF were crystal clear, total protein and sugar concentration ranged from 20 to 400mg/dl and 20 to 180mg/dl respectively. In turbid CSF???s, total protein and sugar concentration varied from 70 to 500mg/dl and 10 to 200mg/dl respectively, while in the highly turbid CSF???s, they ranged from 50 to 800mg/dl and 40 to 140mg/dl respectively. Among total 79 CSF samples, Pandey???s tests were positive for 16.9% and negative for 9.2% in cases of the crystal clear. In case of moderately turbid and highly turbid CSF???s, Pandey???s test was positive for 88.9% and 100% cases respective. C-Reactive protein (CRP) were positive (>12mg/dl) for 3(3.79 %) samples. A total of 79 CSF was culture. There were 5 culture positive cases, which included Escherichia coli (20%), Haemophilus influenzae (20%) and Streptococcus pneumoniae (60%). Using the latex agglutination test, the detection rate was higher than that of culture. Most meningitis positive cases showed an increased total cell counts as well as proteins concentration and decreased serum sugar concentrations. High resistant rate to cotrimoxazole was observed among the invasive isolates. On the other hand, none of these invasive strains showed resistant to ceftriaxone.

Biography :

Mohammad Zakerin Abedin has completed his MPhil degree in Microbiology from Dhaka University, Bangladesh in 2011, MS and BSc (Hon’s) degree in Microbiology from the University of Chittagong in 2004 and 2003 respectively. He is the Assistant Manager (CSSD) of Globex Marketing Company Ltd. He worked a long time as a Research Officer and Microbiologist in the International Centre for Diarrhoeal Disease Research, Bangladesh and Popular Diagnostic Centre Ltd. He has published more than 12 papers in reputed journals and serving as a book writer cum editor of State Medical Faculty of Bangladesh and BTEB. He is the life member of Bangladesh Society of Microbiologist (BSM).

E-mail: zakerin.du2016@gmail.com