Clinical Microbiology: Open Access

Plasmonic Janus hybrids for detection of small metabolites

Joint Event on 25^th Asia Pacific Biotechnology Congress & 3^rd International Conference on Medical and Clinical Microbiology

May 01-02, 2019 Kyoto, Japan

Haiyang Su and Kun Qian

Shanghai Jiao Tong University, China

Posters & Accepted Abstracts: Clin Microbiol

Abstract:

Introduction & Aim: Advanced bio-analytical platforms rely on materials with tailored structural parameters. Matrix materials decide the performance of Laser Desorption Ionization Mass Spectrometry (LDI MS), which may afford fast analysis of analyses in seconds, high sensitivity/resolution, simple sample preparation for practical use. Plasmonic materials are superior candidates for LDI MS use. Particularly, core-shell noble metal hybrids have high yield of hot carriers and surface roughness in the nanoscale. While most current results are based on isotropic core particles, Janus particles are a kind of material with anisotropic structure. Herein, we assembled plasmonic Janus hybrids for LDI MS detection of small metabolites.

Method: We applied Janus particles (Periodic Mesoporous Organosilica (PMO) in conjunction with carbon spheres) as core materials and loaded silver nanoparticles as nanoshells.

Result: Janus core particles enhanced the LDI MS detection of both hydrophobic and hydrophilic molecules due to anisotropic properties. Moreover, plasmonic nanoshells enabled direct metabolites detection with silver adduction in bio-mixtures, due to the selective LDI process. Furthermore, we demonstrated the tunable surface chemistry of Janus hybrids for enrichment and identification of metabolites at low concentration (4 pM).

Conclusion: In summary, we developed a series of Janus hybrid materials for analysis of both hydrophobic and hydrophilic small metabolites, metabolic analysis in complex bio-mixtures and also selective enrichment and detection of target molecules. We anticipated two major research lines along this study, including new bio-nano-interface construction and novel bioanalytical application. This study contributes to the design of materials for high-performance metabolic analysis and clinical diagnostic tools.

Biography :

E-mail: seasunshine@sjtu.edu.cn