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Phagocytosis and killing of Brucella melitensis by phagocytic cells and lymphocytes of goats
Joint Event on 25th Asia Pacific Biotechnology Congress & 3rd International Conference on Medical and Clinical Microbiology
May 01-02, 2019 Kyoto, Japan
M Zamri-Saad and N N Shaqinah
Universiti Putra Malaysia, Malaysia
Posters & Accepted Abstracts: Clin Microbiol
Abstract:
This paper describes the phagocytosis and killing efficiency of the phagocytic cells and lymphocytes of goats. Six adult male goats were divided into two groups. Group-1 was exposed sub-cutaneous to 109 cfu/mL of killed B. melitensis while Group-2 was similarly exposed to sterile PBS. After 2 weeks, blood samples were collected. The neutrophils, monocytes and lymphocytes were isolated and prepared as monolayer. Then, 200 μL of an inoculum containing 107 cfu/mL of live B. melitensis was introduced and incubated at 37 ºC in 5% in 5% CO2. At 0, 30, 60 and 120 minutes of incubation, they were harvested, stained with acridine orange and observed under fluorescence microscope at 1000x magnification. A minimum of 100 cells were examined for the presence and viability of bacteria. Phagocytosis by neutrophils and macrophages showed increasing pattern with time. Earlier exposure significantly (p<0.05) enhanced the phagocytic activity after 60 and 120 minutes by neutrophils and macrophages, respectively. Penetration into the lymphocytes also increased with time and earlier exposure significantly (p<0.05) enhanced the penetration after 60 minutes. There was insignificant (p>0.05) killing efficiency by the neutrophils but the macrophages showed significant (p<0.05) increasing pattern after 60 minutes. Similarly, survival of B. melitensis within the lymphocytes showed insignificant (p>0.05) decreasing pattern. In conclusion, exposure to killed B. melitensis significantly (p<0.05) enhanced the phagocytosis and killing efficiency of macrophages but insignificantly (p>0.05) improved the killing by neutrophils and lymphocytes.
Biography :
E-mail: mzamri@upm.edu.my