Journal of Food Processing & Technology

Optimization of the extraction process of antioxidants from Lotus corniculatus l. using response surface methodology

4^th International Conference and Exhibition on Food Processing & Technology

August 10-12, 2015 London, UK

Milica Zelenika*1, Mirjana Menkovska2, Dragutin Đukic1, Leka Mandic1, Vladeta Stevovic3 and Pavle Maškovic4

Posters-Accepted Abstracts: J Food Process Technol

Abstract:

Different extraction conditions may significantly influence extraction of particular compound groups. In present study
response surface methodology (RSM) based on Box-Behnken design was used to define the best combination of extraction
temperature (20-60°C), ethanol concentration (10-90%) and extraction time (60-180 min) for maximum yield of antioxidant
compounds and maximum antioxidant activity of orange extracts. Experimental values of total phenols yields were in the range
from 4.70 to 5.20 mgGAE/ml, while total flavonoids content was in the range from 2.35 to 2.70 mgRE/ml. Antioxidant activity
expressed as the 50% inhibition concentration (IC50 value) was in the range from 0.055 to 0.095 mg/ml. The experimental results
were fitted to a second order quadratic polynomial model and they have shown a good fit to the proposed model (R2>0.90).
Determined optimized conditions for maximizing yield of antioxidant compounds were within the experimental range.

Biography :

Milica Zelenika is Ph.D student on the Faculty of Agronomy, Cacak, University of Kragujevac from 2014. She graduated academic - Master studies of Biology on the
University of Belgrade - Master of biology 2012, and 2010 finished undergraduate studies of Biology on the University of Belgrade. She visit plenty national and internationa
biological research camps, and work as a researcher or mentor of different Sections. She worked on the project “Empty batteries - filled basket” in the implementation of
the Cultural center Lift from Cacak in the competition “Really important changes,” the Foundation “Ana and Vlade Divac” Fields of interest: Herbal extraction, Antimicrobial
activity of plant extracts.