Clinical Microbiology: Open Access

New genotypes of aflatoxin-producing fungi from Egypt and Philippines

7^th Euro Global Summit on Clinical Microbiology and Mycotoxins

February 27-28, 2017 Amsterdam, Netherlands

Hassan Amra1, Taha Hussien, Yousef Y Sultan, Naresh Magan, Ana Liza Carlobos-Lopez, Christian Joseph R Cumagun and Tapani Yli-Mattila

National Research Centre, Egypt
Cranfield University, UK
University of the Philippines Los Baños, Philippines
University of Turku, Finland

Posters & Accepted Abstracts: Clin Microbiol

Abstract:

Aflatoxins (AFs), mainly produced by Aspergillus flavus and A parasiticus, are the major contaminants of crops in areas with hot climates. These compounds are carcinogenic and act as a cancer initiator. 160 single spore isolates of A flavus/parasiticus were obtained from Egypt and the Philippines. 26.5% of the isolates produced AFs. Producers of four chemotypes of aflatoxin were found. Surprisingly, all positive isolates of A parasiticus produced higher amounts (2400-40400 ng ml-1) of total AFs (AFB1, AFB2, AFG1 and AFG2) than the positive isolates of A flavus (<1200 ng ml-1). All isolates, which were able to produce AFs, gave a positive signal with the ver-1/ver-2 and ordAF/ordAR primers, which amplify ver-1 and ordA genes in the aflatoxin biosynthetic pathway. Based on PCR products of ver-1 gene, new genotypes of aflatoxigenic fungi were found, which revealed the variability of AFs production between different isolates depending on the sources of isolation.

Biography :

Email: tymat@utu.fi