Journal of Advanced Chemical Engineering

Nanostructured materials for multifunctional bioengineering applications

2^nd International Conference on Advances in Chemical Engineering and Technology

November 16-17, 2017 | Paris, France

Prabir Patra

University of Bridgeport, USA

Posters & Accepted Abstracts: J Adv Chem Eng

Abstract:

The talk will focus on the synthesis and functions of nanomaterials in bioengineering applications. The quantitative relationship between electrospun polycaprolactone scaffold fiber diameter and NIH 3T3 fibroblast adhesion and growth kinetics. By varying three important process parameters�??solution concentration, voltage, and collector screen distance�?? different average fiber diameters were obtained. Although 117 nm represented the lowest possible fiber diameter obtainable, these fibers had beads in them. An increase in fiber diameter to428nm led to uniform fibers without any beads. It was found that cell adhesion and growth kinetics are significantly affected as a function of fiber diameter. Beaded scaffolds offered the lowest cell adhesion and minimal growth kinetics despite having the lowest average fiber diameter. Cell adhesion kinetics remained invariant when the average fiber diameter was in the micron range (1,647 nm), whereas cell-growth kinetics were slightly greater than with 900nm scaffolds. We propose that the uniformness of fibers and the average fiber diameter play an important role in modulating cellular attachment and proliferation in electrospun tissue engineering scaffolds. In order to develop scaffolds for tissue regeneration applications, it is important to develop an understanding of the kinetics of cell attachment as a function of scaffold geometry. We will present how the specific surface area of electrospun scaffolds affected cell attachment and spreading. Number of cells attached to the scaffold was measured by the relative intensity of a metabolic dye (MTS) and cell spreading was analyzed for individual cells by measuring the area of projected F-actin cytoskeleton. Last part of the talk will focus on fundamental issues of nanocomposite systems and the challenges and promise associated with the use of electrospun biomaterials in bioelectronics and as biomimetic materials.