Clinical Microbiology: Open Access

Magnetic squashing of circulating tumor cells on plasmonic substrates for ultrasensitive NIR fluorescence detection

Joint Event on 25^th Asia Pacific Biotechnology Congress & 3^rd International Conference on Medical and Clinical Microbiology

May 01-02, 2019 Kyoto, Japan

Ru Zhang, Biao Le, Wei Xu, Kai Guo, Xuming Sun, Haiyang Su, Lin Huang, Jingyi Huang, Ting Shen, Tao Liao, Yongye Liang, John X J Zhang, Hongjie Dai and Kun Qian

Shanghai Jiao Tong University, China

Posters & Accepted Abstracts: Clin Microbiol

Abstract:

Introduction & Objective: Liquid biopsy promises the clinical research and practice, due to its non-invasiveness and efficiency for diagnostic purpose. Particularly, detection of Circulating Tumor Cells (CTCs) in patients' blood plays a paramount role towards the better management of diverse cancer phenotypes. Distinct from traditional biopsy and imaging methods, CTCs offer new insights and opportunities during precise screening, curative assessment, patients staging and metastasis/recurrence studies of cancer. However, it is very challenging for enrichment and downstream analysis of CTCs, owing to their low abundance usually with one CTC mixing in billions of other blood cells. So, we want to develop a novel method for screening circulating tumor cells.

Method: Here, we demonstrated magnetic enhanced capturing of CTCs onto a Plasmonic Gold (pGOLD) chip, through a microfluidic immuno-magnetic method.

Result: Owing to the squashed/flattened morphology of cancer cells by magnetic forces and the resulting close proximity of Near- Infrared (NIR) labels on cells to the pGOLD surface, we observed an ultrahigh NIR fluorescence enhancement of ~50-120 folds, drastically enhancing the ability of CTC detection, imaging and analysis. We conducted fluorescence enhanced, multiplexed protein biomarkers detection of CTCs for cancer cells spiked samples as well as CTCs in cancer patients' blood.

Conclusion: In this study, we demonstrated magnetic enhanced capturing of CTCs onto a pGOLD chip. We combined micro-fluidic immuno-magnetic enrichment of CTCs with on-chip NIR-FE detection. We detected low CTC concentrations down to ~1 cell/ mL with capture efficiency up to ~90%. Mechanical manipulation of cells by magnetic and other forces on plasmonic substrates represents a promising approach to ultrasensitive bio-analytical applications.

Biography :

E-mail: zhrngru@sjtu.edu.cn