Clinical Microbiology: Open Access

Isolation, identification of virulence factors and antibiotic resistance characterization of Candida species in Karachi, Pakistan

4^th International Conference on Clinical Microbiology and Microbial Genomics

October 05-07, 2015 Philadelphia, USA

Hina Tahir

University of Karachi, Pakistan

Posters-Accepted Abstracts: Clin Microbiol

Abstract:

Candida albicans is a commensal and opportunistic pathogenic agent that causes infection in immune compromised individuals. Several attributes contribute to the virulence and pathogenicity of this yeast, including the production of germ tubes formation in serum or plasma bio film production and extracellular hydrolytic enzymes, particularly phospholipase, estrase and hemolysins. This study aimed to investigate the production of phospholipase, hemolysin and bio-film formation activities in HVS and urine clinical isolates. C. albicans and non-albicans both will be compared by the evaluation of their virulence attributes. Candida has been recovered from several body sites in many populations; it is most often recovered from mucocutaneous surfaces of immune compromised patients. C. albicans isolates produce higher amounts of enzymes than C. tropicalis and C. glabrata. Urine and HVS samples will collect from 50 patients. Samples will culture on Sabouraud dextrose agar (SDA) for diagnosis of Candida. After gram staining and germ tube test performance we will subculture on: CHROM agar (CMA) for species identification .Carbohydrate assimilation test will also perform for species identification and confirmation. C. albicans is the most frequent species isolate responsible for fungal urinary tract infections and vulvo-vaginal Candiasis (VVC). However, non-albicans species C. glabrata, C. tropicalis and C. krusei isolates are also reported.

Biography :

Email: Hinamehmood85@yahoo.com