Journal of Clinical & Experimental Pharmacology

Investigation of vitamin D effect(s) on the kidney function in sub-acute paraquat poisoning compared to N-acetyl cysteine

Joint Meeting on International Conference on Pharmacology and Toxicology & 18^th International Conference on Medicinal and Pharmaceutical Chemistry

October 18-19, 2018 Dubai, UAE

Hamed Felegari, Davood Amadimoghadam, Sara Soleimani Asl, Nejat Kheiripour and Akram Ranjbar

Hamadan University of Medical Sciences, Iran
Kashan University of Medical Sciences, Iran

Posters & Accepted Abstracts: Clin Exp Pharmacol

Abstract:

Paraquat is one of the most common herbicides used in agriculture, which can cause very severe toxicity in humans and animals. In this study, we investigated the effect of N-acetyl cysteine and vitamin D on the oxidative toxicity of kidney tissue in sub-acute toxicity with paraquat. 36 male albino rat 8 weeks were randomly divided into 6 groups (n=6). Group 1 or control: Control group (received plate and water), group 2 and 3: Control received with vitamin D dosage in ?g/kg/day2 and N-acetyl cysteine 6.25 mg/kg/ day, group 4: Received paraquat 5 mg/kg/day, group 5 and 6: Poisoned with paraquat received vitamin D-treated dose of 2?g/kg/day and N-acetyl cysteine 6.25 mg/kg/day, this treatment continued for 7 days. At the end of the study, serum and kidney tissue collected. Total Antioxidant Capacity (TAC), lipid Peroxidation (LPO), Total Thiol Groups (TTG), urea and creatinine, vitamin D levels in kidney tissue by spectrophotometric and ELISA methods were evaluated. A kidney histo-pathologic evaluation was performed. The value of p<0.05 was considered significant. In paraquat-poisoned groups lipid peroxidation, urea and creatinine were increased and total antioxidant capacity, thiol groups and vitamin D levels decreased significantly (p<0.05). In treated groups, there was a significant decrease in kidney injury, lipid peroxidation, urea and creatinine in comparison with paraquat group and total antioxidant capacity, thiol groups, vitamin D levels significantly increased (P<0.05). Vitamin D decreased oxidative stress and tissue damage in the kidney caused by paraquat poisoning.

Biography :

Hamed Felegari is a pharmaceutical student from School of Pharmacy Hamedan University of Medical Sciences-Iran. Member of Student Research Committie, University of Medical Sciences, Hamadan, Iran.

E-mail: Dr.hamed.felegari@gmail.com