Clinical Microbiology: Open Access

Introducing a novel device for early detection of breast carcinoma and the potential therapeutics

Joint Event on 17^th International Conference on Pharmaceutical Microbiology and Biotechnology & 23^rd Edition of International Conference on Immunology and Infectious Diseases

April 29-30, 2019 London, UK

Monidipa Ghosh

National Institute of Technology, India

Posters & Accepted Abstracts: Clin Microbiol

Abstract:

In recent years fabrication of immunosensors using screen-printed electrodes (SPEs) gained much importance because of their low cost, portability and re-usability. An electrochemical sensor, based on SPEs is applied for the precise detection of different disease biomarkers and is one of the thrust areas of research, especially in healthcare. Amperometric type immunosensors based on screen printed electrodes has been developed and characterized. Fabricated electrodes were used for the detection of the Human Epidermal Growth Factor Receptor-2 (HER2) antigen. Over expression of HER2 ECD in serum is a key prognostic marker of breast cancer patients. The Screen Printed Electrodes (SPEs) were fabricated and a sandwich enzyme linked immunosorbent assay (ELISA) format was followed for the construction of the immunosensor for the detection of HER2. The detection of HER2 in the serum samples of invasive and non-invasive breast cancer patients has been realized. A novel treatment strategy for breast carcinoma was proposed and evaluated in this study. Silver nanoparticles gained much importance because of their unique physico-chemical properties. Despite their widespread use the mechanism of silver nanoparticles in biocidal activity was not fully understood. Anti-cancer activity of silver nanoparticles (AgNPs) synthesized by cost effective and rapid Glancing Angle Deposition technique (GLAD). Physical analysis revealed well dispersed, spherical, highly crystalline silver nanoparticles. The present study revealed that the potential cytotoxic effect of GLAD AgNPs in MCF-7 cell line by inhibiting growth of cells in a dose dependent and time dependent manner and subsequent DNA fragmentation at IC50 values. The overall results indicated that the GLAD AgNPs have anti-cancer activity through induction of apoptosis in MCF -7 cells, suggesting that GLAD synthesized AgNPs might be a potential alternative agent for the treatment of breast carcinoma. The potentiality of the nanoparticles, as anti-cancer agent has been further validated in patients' breast carcinoma tumour sample cells.

Biography :

E-mail: gmonidipanitd@gmail.com monidipa.ghosh@bt.nitdgp.ac.in