Journal of Food Processing & Technology

Identification of the antimicrobial effect of liquorice extracts on gram-positive bacteria: Determination of minimum inhibitory concentration and mechanism of action using a luxABCDE reporter strain

International Conference on Food Microbiology

August 08-10, 2016 Birmingham, UK

Madiha El Awamie and Catherine Rees

The University of Nottingham, UK

Posters & Accepted Abstracts: J Food Process Technol

Abstract:

The public is demanding foods that are more natural and less processed; this has led to a search for new antimicrobial substances from other sources including plants. Liquorice plant is used as a traditional medicine in different countries around the world to treat many diseases including bacterial infections and also commonly used as flavoring in food products. The aim of this study is to evaluate the anti-bacterial activity of a Glycyrrhiza glabra extract as a natural preservative. This particular extract is derived from the waste material left over after processing of the plant material for food production. Antibacterial activity of liquorice extract was screened against eight species of gram-negative and gram-positive bacteria, including Listeria monocytogenes, Listeria innocua, Staphylococcus aureus, Enterococcus faecalis and Bacillus subtilis. The gram negative bacteria include Pseudomonas aeruginosa, Escherichia coli and Salmonella typhimurium but none of these were affected by the extract. For all of the Gram-positive bacteria tested, growth was inhibited as monitored using optical density, but the cells were not killed since the cells were still viable when plated out. The Minimum Inhibitory Concentration [MIC] and Minimum Bactericidal Concentration [MBC] of the extract was also determined and a concentration of 50 μg ml-1 was found to have a strong bacteriostatic effect on gram-positive bacteria. Microscopic analysis indicated that there were changes in cell shape and results using a bioluminescent reporter strain indicated that cell energy levels were reduced. Together these results suggest that the extract is inhibiting the growth of gram-positive bacteria only by damaging the cell wall and/or membrane.

Biography :

Madiha El Awamie has completed her MSc degree in 2007 at Benghazi University, Faculty of Science, Botany Department Benghazi Libya and worked as Lecturer Assistant in Botany Department at Benghazi University. She is presently a PhD student at Nottingham University, UK.

Email: stxma46@nottingham.ac.uk