Clinical Microbiology: Open Access

Identification of hemagglutinin subtype-specific influenza virus exposures in influenza outbreak investigations by a high throughput 43-plexed MAGPIX platform

7^th Annual Summit on Microbiology: Education, R&D and Market

September 28-29, 2018 | San Antonio, USA

Zhu-Nan Li, Emily Cheng, Eugenie Poirot, Kimberly M Weber, Katharine Sturm-Ramirez, Alicia Fry, James Stevens, Terrence Tumpey and MinZ Levine

National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, USA
Battelle, USA
New York City Department of Health and Mental Hygiene, USA
Centre for Communicable Diseases, Bangladesh

Scientific Tracks Abstracts: Clin Microbiol

Abstract:

The development of serological assays that can rapidly assess the human exposure to novel influenza viruses remains a public health need especially during outbreak investigations. Here, we developed a high throughput 43-plexed magnetic fluorescence microsphere immunoassay (MAGPIX�) combined with serum adsorption technique to identify novel influenza virus exposures. Recombinant hemagglutinins (HAs) from 39 influenza strains representing 8 influenzas A subtypes (H1N1, H2N2, H3N2, H5N1, H7N2, H7N9, H9N2, H13N9), both lineages of influenza B viruses, and a protein A control was used in the 43-plexed MAGPIX. Utilizing the MAGPIX and microneutralization assays (MN), we analyzed 119 single sera collected from animal shelter workers during a 2016-17 A(H7N2) outbreak among cats in New York City (NYC) where the first case of cat-tohuman transmission of A(H7N2) was identified. Paired sera from 29 poultry workers who reported influenza-like illness during an A(H5N1) live bird market surveillance study in Bangladesh were also analyzed. The median fluorescence intensity (MFI) detected by the MAGPIX correlated well with MN titers. The highest correlation coefficient between MFI and MN was achieved to indicate potential influenza virus exposure: Pearson r= 0.63 (n=119) in the NYC A(H7N2) study; and Pearson r= 0.78 (n=58) in Bangladesh A(H5N1) surveillance. In the A(H7N2) study, MFIs against H7 rHA in persons with MN<40 were significantly reduced (p<0.05) following serum adsorption using ectodomain H1 and H3 rHAs compared to those in persons with MN???40, indicating that MAGPIX combined with adsorption can detect A(H7N2)-specific antibodies. For Bangladesh poultry workers, MFIs against H5 rHA did not change after serum adsorption, suggesting that these poultry workers from live bird markets in Bangladesh had been previously exposed to A(H5N1) previously. These results demonstrate that high throughput MAGPIX can be used to identify novel influenza virus exposures during influenza outbreak investigations and in influenza surveillance studies.

Biography :

Zhu-Nan Li has his expertise on influenza virus entry and serology. He has been working on the development of novel serology assays to understand antibody responses following natural infection and vaccination in human and animals.

E-mail: hix7@cdc.gov