Clinical Microbiology: Open Access

High levels of serum angiopoietin-2 and angiopoietin-2/1 ratio at critical stage of dengue hemorrhagic fever patients and their associations with clinical and biochemical parameters

Joint Event on 25^th Asia Pacific Biotechnology Congress & 3^rd International Conference on Medical and Clinical Microbiology

May 01-02, 2019 Kyoto, Japan

Maheshi Mapalagamage, Shiroma Handunnetti, Sunil Premawansa, Rajitha Wickremasinghe, Gayani Premawansa, Karunayokiny Kanaphathippillai and Aruna Dharshan De Silva

University of Colombo, Sri Lanka University of Kelaniya, Sri Lanka Colombo North Teaching Hospital, Sri Lanka Genetech Research Institute, Sri Lanka

Scientific Tracks Abstracts: Clin Microbiol

Abstract:

High vascular permeability is a main feature of severe dengue infection resulting in plasma leakage leading to many adverse effects on patients increasing disease morbidity and mortality. The role of serum Angiopoietin-1 (Ang-1), Angiopoietin-2 (Ang-2) which are known to be associated with endothelial stability in patients with different disease stages were studied. Serum samples were taken from confirmed Dengue Fever (DF) patients on admission (DFA, n=36) and discharge (DFD, n=18); from confirmed Dengue Hemorrhagic Fever patients (DHF) three sets of samples were used; on admission (DHFA, n=36), at critical stage (DHFC, n=26) and on discharge (DHFD, n=18). Age-gender matched healthy individuals were also recruited as controls (HC, n=24). Results showed that Ang-1 and Ang-2 levels are associated with disease status in DHF but not in DF. The highest Ang-2 levels were observed in DHFC samples as compared to DHFA, DFA, DFD and HC (p<0.002 in all comparisons); the lowest Ang-1 levels were observed in DHFC samples compared to DHFA, DHFD and HC (p<0.050 for all). Specifically, the ratio of serum Ang-2/Ang-1 levels in DHFC were the highest compared to all other study categories tested (p<0.001). A significant positive correlation was observed between serum Ang-1 and platelet count in DHFA samples (Pearson r=0.674, p<0.001) and Ang-1 with Pulse pressure in DHFC category (r=0.636, p=0.011). Using a cut-off value of 1.02 for the Ang-2/Ang-1 ratio of samples obtained at the critical stage a sensitivity of 82.6% and specificity of 80.4% discerning DF from DHF was obtained. This signifies the potential use of the serum Ang-2/Ang-1 ratio as a biomarker for DHF critical stage.

Biography :

Maheshi Mapalagamage has completed her BSc in Zoology from University of Colombo, Sri Lanka and is currently pursuing her PhD at Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo. Her PhD is based on investigating prognostic markers for severe dengue infection with respect to oxidative stress and endothelial dysfunction and underling immunopathogenic mechanisms. She is also working as an Assistant Lecturer at Department of Zoology and Environment Sciences, University of Colombo, Sri Lanka.

E-mail: sasika1212@gmail.com