Clinical Microbiology: Open Access

Halomonas Pacifica MML1909, a bioactive potential halophilic bacterium

8^th CLINICAL MICROBIOLOGY CONFERENCE

October 26-28, 2017 | Paris, France

K M Sucharitha, C Ramprasath, G Abirami, R Srinivasan and N Mathivanan

University of Madras, Guindy Campus, India
D G Vaishnav College, India
CSIR-Central Leather Research Institute, India

Posters & Accepted Abstracts: Clin Microbiol

Abstract:

The present study was to evaluate the antioxidant and cytotoxic potential of halophilic bacteria isolated from the Kelambakkam salt pan, TN, India. Totally 18 sediment samples collected from various reservoir ponds and were used to isolate halophilic bacteria. The antioxidant activity measured by DPPH assay followed by confirming with TLC (Thin Layer Chromatography) autobiography. Among 63 halophilic bacterial crude extracts, 2, 7 and 41 extracts exhibited cytotoxicity against A549, HepG2 and MCF-7, respectively at 1 mg/ml in MTT assay. Totally 30 crude exhibited both antioxidant and cytotoxicity. Based on the antioxidant activity and cytotoxicity, 12 halophilic bacterial crude extracts were shortlisted due to their IC50 values against A549, HepG2 and MCF-7 cell lines. The polyphasic taxonomical study revealed the selected effective halophilic bacterial isolate MML1909 is Halomonas Pacifica. The purified crude extract of H. Pacifica MML1909 using column chromatography resulted in 18 fractions. The selected fraction no. 14 further purified by column chromatography followed by preparative TLC. The pure compound characterized by various spectral analyses such as IR, UV, 1H NMR and 13C NMR, with the molecular formula as C20H30N4O6 and molecular weight as 422.48 by ESI-MS. The IC50 value of pure compound of Halomonas Pacifica MML1909 determined as 45 µg and 25 µg on A549 and MCF-7 cell lines respectively. The apoptotic morphological changes were observed by staining with Acridine orange and Ethidium bromide on A549 and MCF-7 cell lines followed by flow cytometry and DNA fragmentation analysis. These results highlighted the importance of Halomonas Pacifica MML1909 as a source for antiproliferative bioactive compounds. Diagnosis of paediatric sepsis by automated blood culture system and conventional blood culture Adeel Ahmad1 Shagufta Iram2 Shahida Hussain3 and Noshin Wasim Yusuf4 University of Health Sciences Lahore, Pakistan Objective: To evaluate the time required for isolation of aerobic bacterial pathogen from paediatric septicaemia suspects by using BACTEC 9240 blood culture system, and to compare the results with conventional blood culture technique. Methods: This comparative cross-sectional study was conducted at the Jinnah Hospital, Lahore, Pakistan, from July to December 2013, and comprised blood samples of suspected septicaemia children. The blood samples were inoculated into automated BACTEC 9240 Peds Plus/F resin-based media. At the same time, conventional blood culture bottle was also inoculated for comparison. The time of culture positivity and bacterial spectrum isolated from these samples was evaluated. SPSS 20 was used for data analysis. Results: Of the 100 blood culture samples, 36(36%) were true pathogens on BACTEC 9240, while 24(24%) were positive through the conventional method. The mean age of the participants was 0.65±20 days. Staphylococcus aureus was detected in 12(12%) samples by BACTEC 9240 and in 7(7%) cases by conventional system. BACTEC 9240 significantly reduced time of positivity from 48 hours to 21 hours compared to conventional system. The number of samples detected within 36 and 48 hours was 7(19.4%) by BACTEC 9240 and 17(70.8%) by conventional system (p<0.05). Conclusion: Diagnosis of paediatric septicaemia through BACTEC 9240 was quicker with high yield and great sensitivity compared to the conventional technique.