Clinical Microbiology: Open Access

Genome sequence and genome mining of a bioactive deep-sea sediment isolated bacterium Amycolatopsis sp.WH001

4^th International Conference on Clinical Microbiology and Microbial Genomics

October 05-07, 2015 Philadelphia, USA

Su Chen

Zhejiang University of Technology, China

Posters-Accepted Abstracts: Clin Microbiol

Abstract:

A deep-sea sediment derived actinobacteria Amycolatopsis sp.WH001 was identified as a broad-spectrum antibiotics producer and shared a 98% 16s ribosomal RNA (rRNA) sequence similarity with that of Amycolatopsis lactamdurans, which can produce cephamycin C. To further evaluate its biosynthetic potential and interesting or unusual features, the complete genome was sequenced with a size of 9,138,610-bp circular chromosome and a 67,043-bp dissociated plasmid. Genome analysis revealed 27 gene clusters potentially encoding secondary metabolite biosynthesis pathways including three polyketide synthases (PKSs), five non-ribosomal peptide synthetases (NRPSs), three hybrid NRPS-PKSs, four terpene, two lantipeptide and several other compounds biosynthetic gene clusters. One of the NRPS gene cluster, nrps3, shared a 57% nucleotide similarity with cephamycin C gene cluster, indicating the capacity of this organism to produce cephamycin C analogue. This study affirmatively identified Amycolatopsis sp.WH001 as a source of NRPS metabolites and highlighted genome mining of interested organism as a powerful tool for natural product discovery.

Biography :

Email: suchen@zjut.edu.cn