Clinical Microbiology: Open Access

GeneXpert: A new tool for the rapid detection of Rifampicin resistance in Mycobacterium tuberculosis

6^th Clinical Microbiology Conference

October 20-22, 2016 Rome, Italy

Muhammad Saeed

Allama Iqbal Medical College, Pakistan

Posters & Accepted Abstracts: Clin Microbiol

Abstract:

This study aimed to evaluate the diagnostic sensitivity and specificity of GeneXpert (MTB/RIF) assay for the detection of Rifampicin (RIF) resistance in Mycobacterium tuberculosis (MTB) by comparing the results with conventional drug susceptibility testing (DST) as �??Gold Standard�?�. A total of 2200 pulmonary and extra-pulmonary specimens were collected from TB suspects from 2012 to 2014. All specimens were processed for ZN staining, LJ culture according to WHO protocol GeneXpert (MTB/RIF) as per manufacturer instructions. All the cases which are positive for MTB were further processed for DST for RIF. Out of 2200 TB suspects, 840 (49.46%) cases were GeneXpert (MTB/RIF) positive for MTB. Among these 15.6% (134/840) cases showed RIF resistance. The sensitivity, specificity, PPV and NPV of GeneXpert for RIF resistance were found to be 98.3%. 99.1%, 94.7% and 99.4% respectively by comparing the results with DST. Our study revealed that GeneXpert (MTB/RIF) is an extremely helpful diagnostic tool for detection of RIF resistance in TB suspects with fairly high sensitivity and specificity along with 2 hours turnout time, which facilitates proper in time management and treatment among MDR-TB patients in developing countries.

Biography :

Email: mian.scientist@yahoo.com