Journal of Food Processing & Technology

Genetic variability of red clover genotypes on the bases of morphological and microsatellite markers

4^th International Conference and Exhibition on Food Processing & Technology

August 10-12, 2015 London, UK

Gordana Surlan-Momirovic

Posters-Accepted Abstracts: J Food Process Technol

Abstract:

Red clover (Trifolium pratense L) is one of the most important sources of animal food. Evaluation of the accessions for
the extent of genetic diversity is of particular importance for red clover breeding programs as well as for conservation of
genetic resources. In the present investigation, 40 diploid and tetraploid red clover genotypes from 17 different countries around
the world were screened for their genetic diversity using 13 morphological traits and 15 microsatellite markers. The traits
analyzed and investigated were 8 quantitative morphological traits and 5qualitative traits. Principal component analysis (PCA)
and applied GGE biplot model for 8 quantitative character data accounted for 83.7% of the total variation of the standardized
data, consisting of 69.2 and 14.5 % of variance attributable to PC1 and PC2 respectively. UPGMA cluster analysis based on the
quantitative morpho-agronomic traits allowed the identification of 6 basic groups with genetic distances ranging from 0.56 to
10.35. Spearman correlation coefficient among red clover quantitative traits varied from 0.25 to 0.96. For analyzed qualitative
traits Shanon diversity index varied from 3.595 to 3.673 with average value 3.631 and UPGMA cluster analysis based on these
type of markers grouped the accessions of red clover into 8 clusters. Microsatellite analysis showed the number of fragments
amplified by each SSR primer combination varied from 24 for RCS3681 to 108 for RCS1729 with an average value of 76.4 per
primer combination. The similarity coefficients based on 15 SSR markers ranged from 0.50 to 0.90 among the 40 accessions
studied. The dendrogram constructed using SSR markers produced ten distinct clusters. The cluster size varied from 1 to 14
genotyps of red clover. Principal coordinates analysis revealed that first (PCoA 1) accounted 5.5% and the second (PCoA 2)
accounted for 5% of the total SSR variation. The analyzed red clover accessions showed significant variation in phenotypic and
SSR characters, indicating high genetic diversity which could allow development of new cultivars with desired traits through
selection and crossing programs.

Biography :

Gordana Šurlan Momirovi�? is professor of Genetics at the Faculty of Agriculture University of Belgrade, Department of Genetics and Plant Breeding. Gordana Šurlan
Momirovi�? has 202 scientific publications, 5 monographs, 2 textbooks, 20 scientific projects and number of citations at SCI list is 140. For her research use methods
in classical and molecular breeding of cereals, comparative genomics of gene families and map-based cloning of resistance genes. She was Visiting Professor at the
University of Missori Columbia, Mo, USA and at Master Academic Study “Environmental Protection” Norwegian University of Life Sciences, Ǻs, Norway. Gordana Šurlan
Momirovi�? is Head of Department for Genetics and Plant Breeding the Faculty of Agriculture University of Belgrade, member of editorial board of Serbian Journal of
Scientific Agricultural Research, member of The Genetics Society of Serbia, Vice-President of Fulbright Alumni Association of Serbia, member of Belgrade University
Council, member of Acreditaion
Commicsion at the Faculty of Agriculture University of Belgrade, President of Commission for International Collaboration, Faculty of Agriculture University of Belgrade,
member of the Fulbright Academy of Science, member of the Board of the Balkan Society for Pedagogy and Education, Greece, president of the Steering Committee of
international project Norway and Serbia “Education, Research and Training for Global Environmental Change and Sustainable Management of Natural Resources in the
Western Balkans”, membership of DAAD (Dauscher Akademscher Austauschdiens) Alumni Association of Serbia.