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4th International Conference on Microbiology

Tokyo, Japan
Expression of TLR-2, 4 and 5 and proinflammatory (IL-6, CXCL-11 and CXCR-3) and antiinflammatory cytokines (IL-10) among patients with irritable bowel syndrome
7th Euro Global Summit on Clinical Microbiology and Mycotoxins
February 27-28, 2017 Amsterdam, Netherlands

Ujjala Ghoshal, Ratnakar Shukla, Prabhat Ranjan and Uday C Ghoshal

Sanjay Gandhi Postgraduate Institute of Medical Sciences, India

Posters & Accepted Abstracts: Clin Microbiol


Evidence of low grade inflammation and altered host-microbial interactions suggests that innate immune response may play an imperative role in the pathogenesis of irritable bowel syndrome (IBS). We aimed to study of TLRs (2, 4 and 5), cytokines (IL-6 and IL-10) and chemokines (CXCL-11 and CXCR-3) expression in colonic biopsies among patients with IBS. Quantitative realtime PCR was used to determine mRNA level of TLRs, cytokines and chemokines in 47 patients with IBS (Rome III criteria) and 25 controls. Expression of TLR-4 and TLR-5 was confirmed at protein level using immunohistochemistry. Of 47 patients with IBS, 20 had constipation (IBS-C), 20 had diarrhea (IBS-D) and 7 unclassified (IBS-U). Expression of TLR-4 and TLR-5 was up-regulated in IBS patients than controls (P=0.013 and P<0.001, respectively). Protein level of TLR-4 and TLR-5 was 4.2 and 6.6-fold higher in IBS-D patients than controls. The mRNA level of IL-6 (P=0.003), CXCL-11 (P<0.001) and CXCR-3 (P<0.001) was higher in IBS patients than controls. Expression of IL-6 (P=0.002), CXCL-11 (P<0.001) and CXCR-3 (P<0.001) was higher, while IL-10 (P=0.012) was lower in IBS-D patients than controls. A positive correlation was found between TLR-4 and IL-6 (P=0.043), CXCR-3 and CXCL- 11 (P=0.047), and CXCR-3 and IL-6 (P=0.003). Stool frequency per week showed positive correlation with mRNA level of TLR-4 (P=0.016) and CXCR-3, inversely correlated with IL-10 (P=0.002). Up-regulations of TLRs (4 and 5), proinflammatory cytokine (IL- 6) and chemokines (CXCL-11 and CXCR-3) and down-regulation of anti-inflammatory cytokine (IL-10) may influence intestinal inflammation in a subgroup of IBS patients.

Biography :

Email: ujjalaghoshal@yahoo.co.in