Clinical Microbiology: Open Access

Evaluation of sperm impairing factor from Escherichia coli as male contraceptive in mouse model

Joint Event on 25^th Asia Pacific Biotechnology Congress & 3^rd International Conference on Medical and Clinical Microbiology

May 01-02, 2019 Kyoto, Japan

Aditi Chauhan and Vijay Prabha

Panjab University, India

Scientific Tracks Abstracts: Clin Microbiol

Abstract:

Men in many countries are ready to share the benefits and burden of family planning equally but till date condom and vasectomy are the only contraceptive methods available for them. Therefore, the hunt for more effective and safer alternatives is still on, wherein, one exploiting rich diversity of microorganisms can be thought of. Earlier in our laboratory, Sperm Immobilizing Factor (SIF) isolated from Escherichia coli has shown admirable spermicidal activity in vitro and excellent contraceptive efficacy in female mice. Thus, the present study was designed to exploit SIF as potential male contraceptive using intra testis as a preferred route of administration. SIF (10, 50, 100, 200 and 400 μg) was administered in the right testis whereas the left testis served as control. The mice were sacrificed at Day 3, 7, 14, 21, 30, 45, 60 and 90 and the results in terms of change in body weight, seminal parameters, tissue somatic indices and histopathology were studied. The weight profile and tissue somatic indices of reproductive as well as of non-reproductive organs showed no significant change in all experimental groups. In case of seminal parameters, inhibition of spermatogenesis was observed in right testis treated with 400 μg of SIF and azoospermia persisted till day 90. Further, histological studies revealed blockage of spermatogenesis in treated side as compared to the non-treated side where normal tissue histology was observed. Hence, SIF when administered intratesticularly can lead to complete azoospermia and holds a potential to be exploited as a male contraceptive in future.

Biography :

Aditi Chauhan has completed her BSc and MSc from Chaudhary Sarwan Kumar Krishi Vishvavidyalya, Palampur. She is currently pursuing PhD from Panjab University, Chandigarh.

E-mail: chauhanaditi75@gmail.com