Clinical Microbiology: Open Access

Evaluation of hospital antibiotic policy implementation at the oncosurgery ward - five yearsâ?? experience

8^th CLINICAL MICROBIOLOGY CONFERENCE

October 26-28, 2017 | Paris, France

Aneta Nitsch Osuch, Patryk Tarka1and Katarzyna Jaroszewska

Medical Univeristy of Warsaw, Poland
Saint Family Hospital, Warsaw, Poland

Posters & Accepted Abstracts: Clin Microbiol

Abstract:

Background: The Hospital Antibiotic Policy (HAP) should be implemented to rationalize the antibiotic use and to decrease the risk of spreading of spreading of resistant bacteria. Although strongly recommended, HAP practices in Poland are not often introduced and evaluated. There are also limited data on the antibiotic consumption patterns at surgery and Oncological Surgery Wards worldwide. Aim: The aim of our study was to describe the antibiotic consumption patterns at the large Oncological Surgery Ward before and after implementation of the HAP. Materials & Methods: We conducted a retrospective analysis of the antibiotic use at the Oncological Surgery Ward in Warsaw (Poland) in years 2011-2015. Calculations were based on daily defined doses (DDDs), DDDs/100 hospitalizations and DDDs/100 person-days. We also analyzed drug utilization rates (DU 90% and DU 100%). Results. After implementation of the HAP the total antibiotic consumption increased (365.55 DDD in 2011 vs 1267,85 DDD in 2015). The similar increasing trend was related to DDD/100 hospitalizations (25.88 in 2011 vs 108.17 in 2015) and DDD/100 person-days (14.22 in 2011 vs 33.18 in 2015). The significant change was observed in antibiotic consumption patterns: the use of amoxicillin with clavulanic acid decreased (64% DDD in 2011 vs 27,6% DDD in 2015, p<0,05) while the use of ciprofloxacin increased (5% DDD in 2011 vs 61% in 2015, p<0,05). The DU100% rate varied from 11 in 2014 to 5 in 2015; while DU 90% rate varied from 2 in 2011 to 5 in 2013-2014. Conclusions: The antibiotic consumption at the hospital requires a constant monitoring. Although the implementation of the HAP did not resulted in the decreased total antibiotic consumption, it provided favorable changes at the antibiotic consumption patterns. Genotyping of Clostridium perfringens in sheep of Kerman province Mehrdad Shamsaddini Bafti, Majid Ezatkhah, Mojtaba Alimolaei and Maryam Amini Razi Vaccine & Serum Research Institute-Kerman Branch, Iran Clostridium perfringens (C. perfringens) is an anaerobic sporadic bacterium that is divided into five types (A-E) based on the secretion of its major toxins. The purpose of this study was to investigate the presence of different types of C. perfringens in sheep of Kerman province. For this purpose, a total of 454 specimens were randomly collected from sheep in Kerman area. After preparing the samples and cultivating, colonies were selected based on the structure of colonies, staining and biochemical tests. In the next step, the isolates were characterized by Multiplex PCR method for the presence of C. perfringens encoding genes. C. perfringens types A(52.5%), B(12.5%), C(20%) and D(15%) were detected from isolates. The results showed that type A of C. perfringens was the dominant type. Also, by examining the dendrogram and the similarity of nucleic acids of C. perfringens alpha toxin gene revealed that the alpha C. perfringens genotype was similar to the standard strain.