Clinical Microbiology: Open Access

Epidemiological study on Rift Valley Fever virus among humans in Taiz Governorate, Yemen

Joint Event on 25^th Asia Pacific Biotechnology Congress & 3^rd International Conference on Medical and Clinical Microbiology

May 01-02, 2019 Kyoto, Japan

Mansour Shayif Abdullah Al-Aesayi

Sana'a University, Yemen

Posters & Accepted Abstracts: Clin Microbiol

Abstract:

Rift Valley Fever Virus (RVFV), which is transmitted to humans by mosquitoes or direct contact with infected animals, is the etiologic agent of Rift Valley Fever (RVF). Using Enzyme Linked Immunosorbent Assay (ELISA), this study was performed to prepare RVFV antigen, which is used in detecting anti-RVFV antibodies, from liver of infected lambs. The study also aimed to study epidemiological prevalence of anti-RVFV IgM and IgG antibodies among humans, which were conducted in the Research Center of Taiz University and Central Health Laboratory, Taiz City, Southwestern Yemen. RVFV antigen was successfully prepared at a titer dilution of 1: 3200 by Chessboard (CB) ELISA assay. However, out of the 234 samples, 154 samples were positive for ELISA assay, of which 49 samples (31.8%) were positive for anti-RVFV IgM and 105 (68.2%) were positive for anti-RVFV IgG antibodies. The antigen was successfully produced and successfully adhered to ELISA plates. Simple and inexpensive methods gave good results. The result can be used to develop and refine predictive database for RVF transmission based on environmental and remote sensing data.

Biography :

E-mail: abushaef@gmail.com