Clinical Microbiology: Open Access

Diversity and function of rhizobacteria isolated from Zanthoxylum armatum DC

Joint Event on 25^th Asia Pacific Biotechnology Congress & 3^rd International Conference on Medical and Clinical Microbiology

May 01-02, 2019 Kyoto, Japan

Nidhi Srivastava, A B Bhatt and Anup Kainthola

HNB Garhwal University, India Modern Institute of Technology, India

Posters & Accepted Abstracts: Clin Microbiol

Abstract:

Zanthoxylum armatum DC. is an endangered medicinal plant, used in toothache, stomachache, diarrhea etc. Rhizosphere is a rich niche of microbes and should be explored for obtaining Plant Growth Promoting Rhizobacteria (PGPR), which enhances the growth of the plant either directly or indirectly. A total of 144 rhizobacteria were isolated from rhizospheric soil of Z. armatum DC. A total of 12 different genera were identified. A total of 29.17% were Bacillus followed by Pseudomonas (21.53%), Klebsiella, Micrococcus (6.25%) each, Staphylococcus, Azotobacter, Flavobacterium and Serratia being 4.86% each, Xanthomonas (3.47%), Alcaligenes and Enterobacter (2.78%) each and Azospirillium (0.69%). Bacillus was dominant in rhizosphere of Z. armatum followed by Pseudomonas sp. All isolates were tested for PGPR properties. A total of 67.36% isolates were found to be positive for IAA production. These isolates produced IAA ranged between 0.693�?�0.83 μg/ml to 73.41�?�1.95 μg/ml. Highest IAA was produced by Pseudomonas sp. (S3KS8) from Site-3 while lowest IAA was produced by Micrococcus sp. (S3UW3) from Site-3. A total of 46.53% isolates were found to be positive for phosphate solubilization. These isolates solubilized phosphate ranging from 21.4±1.02% to 309.1±1.63%. Highest phosphate was solubilized by Bacillus sp. (S2DS7) from site-2 and lowest by Serratia sp. (S3UR1) from site-3. Another important trait of PGPR, that may indirectly influence the plant growth, is the production of ammonia. A total of 63.19% isolates were found to be positive for ammonia production. Among them 32.61% rhizobacteria produced high amount of ammonia followed by 42.39% moderate and 25.0% generated low amount of ammonia.

Biography :

E-mail: nidhisrivastava78@gmail.com