Journal of Clinical & Experimental Pharmacology

Determination of antioxidant activity and phenolic profile of Paliurus spina-christi P. Mill

2^nd International Conference and Exhibition on Pharmacology and Ethnopharmacology

May 02-04, 2016 Chicago, USA

Emre Kayalar, Sevim Kucuk and Fatih Goger

Anadolu University, Turkey

Posters & Accepted Abstracts: Clin Exp Pharmacol

Abstract:

Paliurus spina-christi P. Mill. (Rhamnaceae), also known as �??Christ's thorn, Karaçalı or �?altı�?�, is a perennial thorny shrub of widespread distribution in dry and rocky places in the Mediterranean region and Asia, and the unique species of the genus Paliurus represented in Anatolia. Christ's thorn is used as a diuretic, against diarrhoea and rheumatism in traditional Turkish herbal medicine. The aim of this study was to determine the antioxidant activity and phenolic profile of Paliurus spina-christi. For this purpose plant materials were collected from Eski�?ehir:Bozda�? in Turkey on August 2015. Powdered dried fruits were macerated with methanol at 25°C for 24h. After evaporation and lyofilization steps the extract was analyzed with ABsciex 3200 Q trap LC-MS/MS system. Catechin hydrate, proanthocyanidin B dimer, naringenin-C-diglycoside, 5-caffeoyl quinic acid 5-p-coumaroylquinic acid, rutin, quercetin hexoside and quercetin were found as major compounds. Extract presented strong antioxidant activity with the score of DPPH IC50=0.062 mg/ml. IC 50 of BHT was determined 0.054 mg/ml. Total phenolic content of the extract was found as same 109.54 mgGAE in 1gr extract.

Biography :

Emre Kayalar has completed his PhD from Istanbul University, Dentistry Facuty, Department of Orthodontics in 2014. He began his Postdoctoral studies and is pursuing second Master’s degree in Anadolu University, Faculty of Pharmacy, Department of Pharmaceutical Botany. He has published more than 5 papers in national or international journals. He participated in many national and international congresses with some presentations.

Email: drkayalar@me.com