Clinical Microbiology: Open Access

Detection and inhibition of bacteria on a dual functional silver platform

Joint Event on 25^th Asia Pacific Biotechnology Congress & 3^rd International Conference on Medical and Clinical Microbiology

May 01-02, 2019 Kyoto, Japan

Wei Xu and Kun Qian

Shanghai Jiao Tong University, China

Posters & Accepted Abstracts: Clin Microbiol

Abstract:

Aim: Detection and inhibition of bacteria are universally required in clinics and daily life for healthcare. Developing a dual functional material is challenging and in demand, engaging advanced applications for both defined bio-analysis and targeted bio-toxicity.

Method: We designed magnetic silver nanoshells as a multi-functional platform for detection and inhibition of bacteria. The optimized magnetic silver nanoshells enabled direct Laser Desorption Ionization Mass Spectrometry (LDI MS) based metabolic analysis of bacteria (~10 per μL), in complex bio-fluids. We monitored serum infection process (0-10 h) by statistics towards clinical classification. Moreover, magnetic silver nanoshells facilitated surface adhesion on bacteria due to nanoscale surface roughness and thus displayed long term antibacterial effects. We studied bacteria metabolism with metabolic biomarkers (e.g. malate and lysine) identified during inhibition, showing cell membrane destruction and dysfunctional protein synthesis mechanisms.

Result: In summary, we developed the MPs@SiO2@Ag with rational designed structural parameters as a dual functional platform, bridging metabolism analysis and bacterial toxicity. For metabolism analysis, we performed direct LDI MS for fast detection and early diagnosis towards bacterial induced infection and related classification. For bacterial toxicity, we achieved long term bacteria inhibition and explored the antibacterial process by metabolic analysis.

Conclusion: Herein, our study not only guided design of material based approaches for bio-analysis and bio-toxicity but contributed to bacteria related diagnosis by using specific metabolic biomarkers for sensitive detection and new insights by monitoring metabolomics change of bacteria for antibacterial applications.

Biography :

E-mail: xuwei_15@sjtu.edu.cn