Clinical Microbiology: Open Access

Community-acquired methicillin-resistant Staphylococcus aureus: Characteristics epidemiology, clinical, microbiology and molecular biology; SCCmec gene analysis and VISA in patients infected with Staphylococcus aureus an

6^th Clinical Microbiology Conference

October 20-22, 2016 Rome, Italy

Latre Buntaran

Mayapada Hospital, Indonesia

Scientific Tracks Abstracts: Clin Microbiol

Abstract:

Community Acquired Methicillin Resistant Staphylococcus aureus (CA-MRSA) is a strain of MRSA that can cause infections in patients in the community, in which patients had no previous risk factors for MRSA infection. This study aims to determine and compare the characteristics of epidemiological, clinical and molecular biology of CA-MRSA with HA-MRSA. Of the 311 S. aureus isolates collected from 2 hospitals (RSAB Harapan Kita and RS Siloam Kebun Jeruk) during the period 2009 to 2011, the prevalence of MRSA is 6% and consists of CA-MRSA (2%) and HA-MRSA (4%) , the pattern of infection as follows : SSTI (skin and soft tissue infections): 56%, UTI (urinary tract infection): 17%, RSA (acute rhino sinusitis): 11%, Pneumonia: 6%, Febrile observation: 5% and ILO (wound infection): 5%. The third-generation cephalosporins and quinolones are the antibiotics mostly used in this study. These third-generation cephalosporins are resistant to all isolates of MRSA (CAMRSA and HA-MRSA), whereas quinolone resistant to HA-MRSA but still sensitive to CA-MRSA. The use of antibiotics against infections by S. aureus of 311 isolates showed that the use of antibiotics: Inappropriate 57%, appropriate 43%, adequate 43%, inadequate 57%, oral 79%, parenteral 21%, original 36% and copy product 64%. Furthermore, 11 strains of Staphylococcus aureus were performed by PCR, in which, there is one strain of Community-Acquired MRSA (CA-MRSA) with SCCmec type II, 3 strains of Hospital-Acquired MRSA (HA-MRSA) with SCCmec type IV and two strains of Hospital-Acquired MSSA (HAMSSA) and five strains of Community Acquired MSSA (CA-MSSA) that do not contain mecA genes and SCCmec. From the three strains; one strain of CA-MRSA and two strains of HA-MRSA containing plasmid pUB110. vraA present in 91% of the 11 strains, vraF: 36%, vraG: 45% and vraR: 36%. Noteworthy, strains without pUB110 contained in a relatively high frequency of 75% in vraR as well as vraF and 70% in vraA compared to strains with pUB110: 60% in vraG.

Biography :

Latre Buntaran has completed his Clinical Microbiologist Specialist studies in 1996 at University of Indonesia and Postdoctoral studies from Hasanuddin University in 2012. He is the Head of Microbiology Department as well as Head of Infection Control Committee at Mayapada and Bethsaida Hospitals. He has published 4 papers in reputed journals and was a speaker in more than 200 national symposiums and several international congresses.

Email: latrebuntaran@yahoo.com