Clinical Microbiology: Open Access

Co-circulation of Echovirus 6 and 30 with Coxsackievirus A6 in an outbreak of hand, foot and mouth disease in Ahvaz, Southwest of Iran

7^th Euro Global Summit on Clinical Microbiology and Mycotoxins

February 27-28, 2017 Amsterdam, Netherlands

Mojtaba Rasti

Ahvaz Jundishapur University of Medical Science, Iran

Posters & Accepted Abstracts: Clin Microbiol

Abstract:

The aim of this study is to evaluate the etiologic agents of hand, foot and mouth disease (HFMD) outbreak in Ahvaz, Southwest of Iran and their evolutionary analysis by phylogenetic construction and simplot analysis. Sixteen (16) serum samples were collected in an outbreak of HFMD in Ahvaz in October 2013. The RNA were extracted from these samples and subjected to reverse transcription polymerase chain reaction for detection of enterovirus group A and B. Positive cases were sequenced and subjected to phylogenetic and simplot analysis for detecting signs of recombination. Among the 16 specimens, 9 (56.25%) were PCR positive with the universal primers for enterovirus 5�??UTR region. Coxsackievirus A6 was detected as a predominant agent of HFMD with two cases of echovirus 6 and 30 by VP3-VP1 and VP1-2A regions sequencing. In case of echovirus 6, signs of recombination in 5'UTR region were observed based on phylogenetic and simplot analysis of 5'UTR and VP1 regions. In conclusion, Coxsackievirus A6 is the main agent of HFMD in Ahvaz. Evidence of recombination of echovirus 6 in this study illustrates the importance of enhancing common hygiene as well as sanitation to prevent the circulation of this strain in community and advent of new strains.

Biography :

Email: mojtabarasti@yahoo.com