Journal of Clinical & Experimental Pharmacology

Chemical composition, antioxidant capacity, and antibacterial activity of essential oils of leaves of Eucalyptus globulus plant

Co-organized Event International Conference on Toxicology and Clinical Pharmacology & 2^nd International Conference on Generic Drugs and Biosimilars

December 14-16, 2017 Rome, Italy

Boulekbache Makhlouf L, Grenier D, Bey Ould Si Said Z, Harkat Madouri L, Rigou P, Allalou H, and Madani K

University of Bejaia, Algeria
Laval University, Canada
University of Sciences and Technology Houari Boumediene, Algeria
INRA, France

Posters & Accepted Abstracts: Clin Exp Pharmacol

Abstract:

The aim of the present study was to investigate the chemical composition of essential oils extracted from leaves of Eucalyptus globulus (E. globulus) plant, using gas-chromatography coupled with mass spectrometry (GC/MS) method, and to evaluate their antioxidant capacity by means of 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, reducing power test, and β-carotene/linoleic acid method, as well as their antibacterial activity against the major Gram-negative periodontopathogenic (Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis) and Gram-positive cariogenic (Streptococcus mutants, Streptococcus sobrinus) bacterial species. The GC/MS analysis allowed the identification of 26 compounds with the predominance of oxygenated monoterpenes (78.58%); 1,8-cineole (55.29%), spathulenol (7.44%) and α-terpineol (5.46%) being the main components. The tested oil exhibited a weak antioxidant capacity, but a marked antibacterial activity against Gram negative bacteria, more specifically F. nucleatum ATCC 25586 (MIC=1.14 mg/mL) and Porphyromonas gingivalis ATCC33277 (MIC=0.28 mg/mL). Therefore, essential oils from E. Globulus may have a potential therapeutic application for the treatment of periodontal diseases.

Biography :

Boulekbache Makhlouf L has her expertise in the extraction, characterization and evaluation of the biological activities of natural substances for their use in the food and pharmaceutical industries. She is a Team Leader in the research laboratory: Laboratory of Biomathematics, Biophysics, Biochemistry, and Scientometrics (L3BS) at the University of Bejaia in Algeria and responsible of the doctoral training: Bio-resources, Environment and Technology Agro-food.