Clinical Microbiology: Open Access

Bioconversion of paper sludge to ethanol by SSF using a novel enzymatic extract produced by filamentous fungi (Verticillium and Penicillium species)

3^rd Global Microbiologists Annual Meeting

August 15-17, 2016 Portland, Oregon, USA

Ivonne Angulo De Castro and Rosa Erlide Prieto Correa

Universidad de La Sabana, Colombia

Posters & Accepted Abstracts: Clin Microbiol

Abstract:

Paper sludge has been considered an attractive and convenient biomass source for second generation biofuels, likewise enzymes are critical factor in bioconversion of lignocellulosic waste, besides this, the cost presents an economical bottleneck in the bioethanol production. An enzymatic extract produced from Verticillium sp. and Penicillium sp., composed of proteins with molecular weight among 11.62-107.6 kDa, was used to produce fermentable sugars via enzymatic hydrolysis and was compared with sugars produced with commercial enzyme Cellic®. Factors as hydrolysis time, temperature, enzymatic extract or enzyme concentration and solid load were evaluated. The hydrolysis process with the extract produced 26% and 17% of reducing sugars than produced with enzyme Cellic® at 37 °C and 45 °C respectively, under the same conditions (18,17% solid loading, 6% extract/enzyme loading, 150 rpm and 12 hours), buffer effect is observed because of ash present in the paper sludge. The best conditions for fermentable sugars production were used to develop an SSF process with Saccharomyces cerevisiae, the process was carried out during 9 days, allowing the production of 14.60±7.4 and 11.18±1.9 g/L of ethanol with enzymatic extract and enzyme Cellic® respectively, corresponding to a theoretical ethanol yield based on initial hexose of 43.12% (g ethanol/g hexose) with enzymatic extract and 33.03% with enzyme Cellic® at day 9 of process. This result shows the potential of the enzymatic extract degrading lignocellulosic material for the production of second generation ethanol, considering that is a crude extract that has not been purified.

Biography :

Email: fsh.gholami@gmail.com