Journal of Clinical & Experimental Pharmacology

Assessment of antibiotic resistance-dissemination from MDR E. coli to ratâ??s microbiota by Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrophotometry (MALDI TOF-MS)

2^nd International Conference and Exhibition on Pharmacology and Ethnopharmacology

May 02-04, 2016 Chicago, USA

Aymn T Abbas, Muhammad Yasir, Imran Khan, Esam I Azhar, Elie K Barbour and Taha Kumosani

King Abdulaziz University, KSA
Mansoura University, Egypt
American University of Beirut, Lebanon

Posters & Accepted Abstracts: Clin Exp Pharmacol

Abstract:

The increasing prevalence of multi-drug resistant (MDR) Escherichia coli is one of the intractable health obstacles of 21st century. Being a part of intestinal microbiota, E. coli could potentially contribute to increasing antimicrobial resistance of gut microbiota (GM). In this study, we aimed to determine the contributing effects of MDR E. coli on antimicrobial resistance of GM in rat model. Ten rats, divided in two groups (groupâ??A and groupâ??B), were orally administered (1 Ã? 105 CFU) with MDR E. coli. The ingested strain was susceptible to amoxicillin. Groupâ??B was treated with amoxicillin to observe MDR E. coli persistence in gut and antimicrobial resistance diversity in GM. The stool samples were aerobically cultured at 37°C. The isolates were screened for resistance against ten different antibiotics and finally identified through MALDI TOF MS and 16S RNA sequencing. Around 8020 colonies were processed. Ratâ??s GM possessed resistance against amoxicillin, D-cycloserin, gentamycin, carbenicillin and kanamycin. The genus Enterococcus dominantly inhabited ratâ??s gut. The ingested MDR E. coli significantly (p=0.01) increase CFU in antibiotics supplemented plates and rose diversity of resistant species. However, the E. coli and resistant CFU count declined seven days after administration. E. coli could not root permanently and was found to be absent 14 days post-treatment. Amoxicillin successfully treated out ingested MDR E. coli and reduced the bacterial load however, the number of resistant species increased in comparison to control. In conclusion, both E. coli ingestion and amoxicillin treatment increased resistance in ratâ??s GM. The ingestion of MDR E. coli followed by amoxicillin treatment increased resistant CFU and leaded to emergence of new antimicrobial resistant bacteria.

Biography :

Aymn T Abbas has completed his PhD from Zagazig University. He is Associate Professor of Veterinary Medicine, Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, KSA. He has published more than 25 papers in reputed journals.

Email: aymntalat2001@yahoo.co.uk