Clinical Microbiology: Open Access

Anti-Salmonella, cytotoxicity and GC-MS analysis of leaves extracts of Carica papaya

Joint Event on 25^th Asia Pacific Biotechnology Congress & 3^rd International Conference on Medical and Clinical Microbiology

May 01-02, 2019 Kyoto, Japan

Abdulrazak M H and Mujaheed A

Sule Lamido University, Nigeria

Posters & Accepted Abstracts: Clin Microbiol

Abstract:

Shed dried matured leaves of Carica papaya were extracted with water, ethanol and chloroform solvents via percolation method. The composition of bioactive compounds in the leaves extracts of Carica papaya was determined by gas chromatography-mass spectrophotometry. The toxicity status of the extracts was tested using Brine Shrimp Lethality assay and bioactivity test was carried out on the bioactive TLC fractions. From the GC-MS study 18 compounds were identified, with oleic acid (36.65%), palmitic acid (16.68%), stearic acid (9.78%) and 9-octadecenal (7.44%) being the major components. The toxicity study revealed that chloroform extracts of C. papaya was not toxic with LC50 value of (11,641 μg/ml), while water extracts have LC50 values of (57.450 μg/mL) and ethanolic extracts presents LC50 values of (179.505 μg/mL), respectively. Lastly results of antibacterial activity using contact bio-autography showed that the only bioactive fractions (Rf2 and Rf3 of chloroform extracts) were active against Salmonella Typhi and Salmonella Paratyphi B only.

Biography :

E-mail: khsabdul@gmail.com