Clinical Microbiology: Open Access

Antibacterial and anti-biofilm effect of Centella asiatica to periodontal pathogenic bacteria, Porphyromonas gingivalis

Joint Event on 17^th International Conference on Pharmaceutical Microbiology and Biotechnology & 23^rd Edition of International Conference on Immunology and Infectious Diseases

April 29-30, 2019 London, UK

Kittipong Laosuwan, Fujita M and Jung H S

The University of Hong Kong, Hong Kong Health Sciences University of Hokkaido, Japan

Posters & Accepted Abstracts: Clin Microbiol

Abstract:

Aim: The aims of this study are to investigate the abilities of Centella asiatica crude extract preventing the Porphyromonas gingivalis (P. gingivalis) biofilm formation and development and the ability to neutralize the virulent factors gingipain and fimbriae function.

Method: The P. gingivalis (strain ATCC33277) was cultured in suspension with TSB medium supplement with vitamin, hemin, menadione and yeast extract. The aqueous extraction was performed to crudely extract from Centella asiatica leaves with pure water, the crude extract was filtered and freeze dried before dissolved in PBS at concentration 0.125, 0.25, 0.5, 1, 2.5, 5, 10 mg/ml. The minimum inhibitory concentration (MIC) was determined for P. gingivalis in planktonic stage. The P. gingivalis biofilm was form in 96-wells plate with chemically defied medium for determining the biofilm formation inhibition effect and inhibitory effect on biofilm development by treating the bacteria before or during the biofilm formation respectively. The biofilm volume was measured with crystal violet assay. The toxicity to immortalized human periodontal ligament cell (ihPDL cell) was determined with MTT assay.

Result: The extract had no toxicity to ihPDL cell at all tested concentration (P>0.05). The extract was able to inhibit the P. gingivalis planktonic growth at concentration of 10 mg/ml. The biofilm volume was significantly reduced at concentration of 2.5 mg/ml for biofilm formation inhibition effect and at 0.25 mg/ml for inhibitory effect on biofilm development (P<0.05).

Conclusion: The Centella asiatica crude extract has a potential to inhibit biofilm formation and development even at lower concentration than MBC. This indicates that the extract might have alternative ways to inhibit the biofilm formation.

Recent Publications

1. Laosuwan K, Epasinghe D J, Wu Z, Leung W K, Green D W and Jung H S (2018) Comparison of biofilm formation and migration of Streptococcus mutans on tooth roots and titanium miniscrews. Clinical and experimental dental research 4(2):40-47.

Biography :

Kittipong Laosuwan is currently pursuing his PhD in the Faculty of Dentistry at The University of Hong Kong. He has interest in microbiology especially developing an alternative medicine from local herb to fight against oral pathogenic bacteria. As a fellow dentist, he aims to develop a medical modality to improve oral health not only treatment but also the prevention for better life.

E-mail: u3002628@hku.hk