Journal of Food Processing & Technology

Antibacterial activity of two wild mushrooms, Amanita echinocephala and Cerena unicolor, collected in Macedonia

4^th International Conference and Exhibition on Food Processing & Technology

August 10-12, 2015 London, UK

Daniela Nikolovska Nedelkoska*1, Natalija Atanasova-Pancevska2, Mitko Karadelev2, Dragan Damjanovski1 and Dzoko Kungulovski2

Posters-Accepted Abstracts: J Food Process Technol

Abstract:

Considering an increasing number of bacteria developing resistance to commercial antibiotics, the unexplored field of
wild growing mushrooms hold great promise as potential new source of natural antimicrobial substances. In this study,
antimicrobial potential of the methanolic extracts of two wild macro-fungi, Amanita echinocephala and Cerena unicolor, was
evaluated. Testing was conducted against six microorganisms, including four strains of Gram-positive bacteria (Bacillus subtilis,
Bacillus pumilus, Sarcina lutea, Staphylococcus aureus) and two strains of Gramnegative bacteria (Pseudomonas aeruginosa,
Escherichia coli). The minimum bactericidal concentration (MBC), as the lowest concentration of the extract required to kill
a particular bacterium, was determined using the microdilution method and subculturing to agar plates that not contain the
test extract.Analyzed mushroom extracts exhibit antimicrobial activity. The extracts from lignicolous mushroom C. unicolor
demonstrated more potent bactericidal effects. Gram-positive bacteria B. subtilis, B. pumilus and S. aureus were most sensitive
to the examined C. unicolor extract (MBC = 1,563 mg/ml).

Biography :

Daniela Nikolovska Nedelkoska has completed her PhD thesis related to the antioxidant properties, bioactive compounds and therapeutic potential of the wild-growing
macrofungi (mushrooms) in 2014. Her competences ranges from food engineering and biotechnology, to biological area of expertise. She has participated in several
national and international projects in her field of interest. Ass. Professor Daniela Nikolovska Nedelkoska has published over 25 original scientific articles and abstracts.