Clinical Microbiology: Open Access

Analysis of β-lactamase genes in multidrug-resistant Acinetobacter baumannii isolated from a hospital in the southwest of China

6^th Clinical Microbiology Conference

October 20-22, 2016 Rome, Italy

Ying Shun Zhou, Jin Ping Zhang, Jing Zeng, Ze Cai Wu and Guang Xi Wang

Southwest Medical University, China

Posters & Accepted Abstracts: Clin Microbiol

Abstract:

Acinetobacter baumannii is an opportunistic pathogen of hospital-acquired infection. The aim of this study was to analysis the β-lactamases genes prevalence in Acinetobacter baumannii isolated from a hospital in the southwest of China. In this study, 87 A. baumannii strains isolated from a hospital in southern of Sichuan province that were tested for antibiotic susceptibility performed by determination the MIC, molecular genotyping by multilocus sequence typing (MLST) and more importantly, the prevalence of β-lactamases genes (including blaOXA-23, blaOXA-24, blaOXA-58, blaGIM, blaSPM, blaSIM, blaNDM, blaCTX-M, blaSHV and blaTEM) were studied by PCR method. Antibiotic susceptibility test against 13 commonly used antibiotics indicated that most of the stains showed multidrug-resistance. And the result of MLST analysis showed that there were 5 STs in the 87 A. baumannii isolates and a majority of them was ST195 (69/87). The PCR amplication and sequencing results illustrated that most of these isolates were positive to blaOXA-23 and blaTEM genes and the prevalence of them were 74.71% and 93.10% respectively. In addition, 1 positive strain for the blaGIM, 1 positive strain for the blaSIM, 1 positive strain for the blaSHV and 2 positive strains for the blaSPM were found. However, all of the isolates were negative to the rest β-lactamases genes detected including blaOXA-24, blaNDM and blaCTX-M. In conclusion, the 87 A. baumannii isolates in this study showed severe drug resistance and the main β-lactamases genes carried were blaOXA-23 gene and blaTEM gene.

Biography :

Ying Shun Zhou has completed his PhD from Sichuan University. He presently works in the Southwest Medical University and is the Director of Pathogenic Microbiology Laboratory. He has published more than 10 papers in reputed journals and has been serving as a Member of Chinese Medical Association.

Email: yingshunzhou@163.com