An amperometric biosensor based on nanocomposite titania-nafion for determining laccase phenol compounds
International Conference on Food Safety and Regulatory Measures
August 17-19, 2015 Birmingham, UK

Martínez-Flores H E, Garnica-Romo M G and Romero-Arcos M

Universidad Michoacana de San Nicolas de Hidalgo, Mexico

Posters-Accepted Abstracts: J Food Process Technol


During the present investigation, an amperometric biosensor for the detection of phenol compounds was developed. Said work was based on the immobilization of the enzyme laccase enzyme in a composite of titania and nafion nanoparticles. The immobilization stage was carried out and conducted by means of a mixture of titania-nafion (v/v) nanoparticles in a ratio 1:1 in which the enzyme was added and subsequently doping realized on the surface of a graphite electrode. Biosensor response was determined by direct reduction of the o-quinone (the product of the enzymatic reaction) at 100 mV in Ag/AgCl (3M KCl) solution. The biosensor was tested by cyclical electrochemical impedance spectroscopy and voltammetry. It was found that the biosensor demonstrated promising analytical results in terms of both sensitivity and linear range for catechol, yielding values of 384 mA L mol-1 and 4-8 mmol/L respectively. The detection limit for catechol was 2 mmol/L. The nanoparticles of titania generated a biocompatible microenvironment for the enzyme producing a compact film with high thermal stability together with extensive superficial area reflected in good catalytic activity for the detection of phenol compounds.

Biography :

Hector Eduardo Martinez-Flores completed his PhD (1997) in the CINVESTAV-IPN Institution, Mexico. He undertook Post-doctoral studies in the Universidade Estadual of Campinas, Campinas, Sao Paulo, Brazil from 1997 to 2000. He was visiting Professor at Washington State University from 2013 to 2014. He has published more than 37 papers in reputed scientific journals, and has been and is serving as reviewer of 18 JCR journals